06-Dec-2017 - University of California, Davis

Microscope using UV instead of visible light emerging as diagnostic tool

MUSE image of sebaceous glandA microscope using ultraviolet light to illuminate samples enables pathologists to assess high-resolution images of biopsies and other fresh tissue samples for disease within minutes, without requiring the time-consuming preparation of conventional slides or destroying the tissue.

This approach holds promise for improving the speed and efficiency of patient care and medical research nationwide, a study has found.

The technology, known as microscopy with UV surface excitation, or MUSE, uses ultraviolet light at wavelengths below the 300 nanometer range to penetrate the surface of tissue samples by only a few microns (about the same thickness of tissue slices on traditional microscope slides.) The phenomenon was originally described by Stavros Demos, one of the co-authors, who is now at the University of Rochester.

Samples that have been stained with eosin or other standard dyes to highlight important features such as nuclei, cytoplasm and extracellular components produce signals from the UV excitation that are bright enough to be detected by conventional color cameras using sub-second exposure times. The process allows for rapid imaging of large areas and immediate interpretation.

Richard Levenson with MUSE technology"MUSE eliminates any need for conventional tissue processing with formalin fixation, paraffin embedding or thin-sectioning," said Richard Levenson, professor and vice chair for strategic technologies in the Department of Pathology and Laboratory Medicine at UC Davis and senior author of the study.

"It doesn't require lasers, confocal, multiphoton or optical coherence tomography instrumentation, and the simple technology makes it well suited for deployment wherever biopsies are obtained and evaluated," he said.

MUSE's ability to quickly gather high-resolution images without consuming the tissue is an especially important feature.

"It has become increasingly important to submit relevant portion of often tiny tissue samples for DNA and other molecular functional tests," he said. "Making sure that the submitted material actually contains tumor in sufficient quantity is not always easy and sometimes just preparing conventional microscope slices can consume most of or even all of small specimens. MUSE is important because it quickly provides images from fresh tissue without exhausting the sample."

The ability to obtain instant, high-resolution, full-color images for histology, pathology or toxicology studies is also useful for basic scientists who want to assess tissue samples from experimental animal models at the laboratory bench. The technology is being commercialized by MUSE Microscopy Inc .

Facts, background information, dossiers
  • tissue
  • biopsies
  • ultraviolet light
More about UC Davis
  • News

    Biological enzymes as source of hydrogen fuel

    Research from the University of Illinois and the University of California, Davis has chemists one step closer to recreating nature's most efficient machinery for generating hydrogen gas. This new development may help clear the path for the hydrogen fuel industry to move into a larger role i ... more

    Breakthrough in designing a better Salmonella vaccine

    UC Davis researchers announce a breakthrough in understanding which cells afford optimal protection against Salmonella infection--a critical step in developing a more effective and safe vaccine against a bacterium that annually kills an estimated one million people worldwide. Professor Step ... more

    Newly discovered enzyme is 'firing pin' for plant immunity

    Just like humans, plants have an immune system that helps them fight off infections. Plant immunity has some important differences: they don't make antibodies and can't fight off the same bug more quickly months or years later. However, plant cells can identify pathogens and react to them, ... more

  • Videos

    UC Davis Researchers Win Grant that Could Help Fight Cancer

    Experts in biomedical engineering and radiology have been awarded $15.5 million from the National Institutes of Health to build a PET scanner that could allow physicians to see inside the entire human body at once. Such a technology could have major implications for how cancer and other dis ... more