Caterpillars as a replacement for mammalian models in preclinical research

Research team developed an innovative and unique imaging platform

06-Dec-2022 - Germany

Inflammation research is becoming more ethical and economical: caterpillars help to understand gut inflammation and enable the faster and more efficient development of new therapies.

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Insects, like caterpillars, suffer from the same or related diseases as humans because 75 % of genes associated with human illness are also present in insects. Consequently, caterpillars like the tobacco hornworm can be used as a model organism, improving the understanding of diseases and helping to test and develop new therapies or diagnostic tools. A team around Anton G. Windfelder, a zoologist at the University of Giessen and employee of Fraunhofer IME, Germany, are reporting the alternative use of caterpillars to study chronic inflammatory bowel diseases in Nature Communications.

"Inflammatory diseases of the alimentary tract affect millions of people worldwide, and the incidence of chronic inflammatory bowel diseases is rising globally," says Dr. Windfelder. "The innate gut immunity and the organization of the intestinal epithelium of tobacco hornworm larvae are comparable to those of mammals. However, in contrast to other insects such as Drosophila, the fruit fly, caterpillars of tobacco hornworm are large enough for medical imaging." Together with national and international cooperation partners from Düsseldorf, Germany, and New York, USA, Anton G. Windfelder and team developed an innovative and unique imaging platform. "We use medical imaging modalities like computed tomography (CT), magnetic resonance imaging (MRI), and positron emission tomography (PET) to characterize a gut inflammation in tobacco hornworm." says Gabriele Krombach, Head of the Clinic for Diagnostic and Interventional Radiology at UKGM Giessen, who is a co-author of the study.

"Intestinal inflammation in larvae of tobacco hornworm is diagnosed using contrast media and tracers such as FDG (18F-desoxyglucose)," says Dr. Windfelder. "This is in analogy to the diagnosis of gut inflammation in humans." Anti-inflammatory medications such as cortisone, used in acute flares of human chronic inflammatory bowel disease, also show a significant reduction in gut inflammation in the larvae of the tobacco hornworm. The advantage of the imaging platform is that multiple animals can be studied simultaneously. Up to 100 animals can be examined in a few seconds using CT. In contrast to traditional methods, the animals stay alive and survive. "The animals tolerate the anesthesia and imaging very well and can continue to live after the experiments."

With the publication mentioned, Windfelder and team want to draw attention to the successful alternative use of insects in early preclinical research. "Of course, insects like cannot fully replace mice and rats.” adds Andreas Vilcinskas, head of the Fraunhofer IME in Giessen and head of the Institute for Insect Biotechnology at the JLU Giessen, who was also involved in the study. "Evidence from cell culture is often not reproducible in in-vivo systems like mice," Vilcinskas says. "Here, insects like Manduca can help identify promising new targets or therapies worth further investigating in traditional mouse models." This would speed up research and make it more affordable. In this way, researchers could significantly reduce the use of classic laboratory animals and thus contribute substantially to animal welfare.

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