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Gli2



GLI-Kruppel family member GLI2
Identifiers
Symbol GLI2
Entrez 2736
HUGO 4318
OMIM 165230
RefSeq NM_005270
UniProt P10070
Other data
Locus Chr. 2 q14

Gli2 is a transcriptional activator and repressor of which there are four isoforms; Gli2 alpha, beta, gamma and delta.[1] C-terminal transcriptional activator and N-terminal repressor regions have been identified in both Gli2 and Gli3.[2] However, the N-terminal part of human Gli2 is much smaller than its mouse or frog homologs, suggesting that it may lack repressor function. Gli2 affects ventroposterior mesodermal development by regulating at least three different genes; Wnt genes involved in morphogenesis, Brachyruy genes involved in tissue specification and Xhox3 genes involved in positional information.[3] The anti-apoptotic protein BCL-2 is up regulated by Gli2 and, to a lesser extent, Gli1 – but not Gli3, which may lead to carcinogenesis[4]. It has been shown in mouse models that Gli1 can compensate for knocked out Gli2 function when expressed from the Gli2 locus. This suggests that in mouse embryogenesis, Gli1 and Gli2 regulate a similar set of target genes. Mutations do develop later in development suggesting Gli1/Gli2 transcriptional regulation is context dependent.[5] Gli2 and Gli3 are important in the formation and development of lung, trachea and oesophagus tissue during embryo development.[6] Studies have also shown that GLI2 plays a dual role as activator of keratinocyte proliferation and repressor of epidermal differentiation.[7]

Additional recommended knowledge

There is a significant level of cross talk and functional overlap between the Gli TFs. Gli2 has been shown to compensate for the loss of Gli1 in transgenic Gli1-/- mice which are phenotypically normal.[8] However, loss of Gli3 leads to abnormal patterning and loss of Gli2 affects the development of ventral cell types, most significantly in the floor plate. Gli2 has been shown to compensate for Gli1 ventrally and Gli3 dorsally in transgenic mice.[9] Gli2 null mice embryos develop neural tube defects which, can be rescued by overexpression of Gli1 (Jacob and Briscoe, 2003). Interestingly Gli1 has been shown to induce the two GLI2 α/β isoforms.

Transgenic double homozygous Gli1-/- and Gli2-/- knockout mice display serious central nervous system and lung defects have small lungs, undescended testes, and a hopping gait as well as an extra postaxial nubbin on the limbs.[10] Gli2-/- and Gli3-/- double homozygous transgenic mice are not viable and do not survive beyond embryonic level.[11][12][13] These studies suggest overlapping roles for Gli1 with Gli2 and Gli2 with Gli3 in embryonic development.

Interestingly, transgenic Gli1-/- and Gli2-/- mice have a similar phenotype to transgenic Gli1 gain of function mice. This phenotype includes failure to thrive, early death, and a distended gut although no tumors form in transgenic Gli1-/- and Gli2-/- mice. This could suggest that overexpression of human Gli1 in the mouse may have led to a dominant negative rather than a gain-of-function phenotype.[14][15]

Transgenic mice over-expressing the transcription factor Gli2 under the K5 promoter in cutaneous keratinocytes develop multiple skin tumours on the ears, tail, trunk and dorsal aspect of the paw, resembling those of basal cell carcinoma (BCC). Unlike Gli1 transgenic mice, Gli2 transgenic mice only developed BCC-like tumors. Transgenic mice with N-terminal deletion of Gli2, developed the benign trichoblastomas, cylindromas and hamartomas but rarely developed BCCs.[16] Gli2 is expressed in the interfollicular epidermis and the outer root sheath of hair follicles in normal human skin. This is significant as Shh regulates hair follicle growth and morphogenesis. When inappropriately activated causes hair follicle derived tumors, the most clinically significant being the BCC.[17]

Of the four Gli2 isoforms the expression of Gli2beta mRNA was increased the most in BCCs. Gli2beta is an isoform spliced at the first splicing site which contains a repression domain and consists of an intact activation domain. Overexpression of this Gli2 splice variant may lead to the upregulation of the Shh signalling pathway, thereby inducing BCCs.[18]

In human keratinocytes Gli2 activation upregulates a number of genes involved in cell cycle progression including E2F1, CCND1, CDC2 and CDC45L. Gli2 is able to induce G1–S phase progression in contact-inhibited keratinocytes which may drive tumour development.[19]

Although both Gli1 and Gl12 have been implicated it is unclear whether one or both are needed for carcinogenesis. However, due to feed back loops, one may directly or indirectly induce the other.

References

  1. ^ Tojo et al., 2003a
  2. ^ Sasaki et al., 1999
  3. ^ (Altaba et al., 2003) II.23
  4. ^ Regl et al., 2004
  5. ^ Regl et al., 2004
  6. ^ Motoyama et al., 1998
  7. ^ Regl et al., 2004a
  8. ^ Ding et al., 1998
  9. ^ Litingtung and Chiang, 2000
  10. ^ Park et al., 2000
  11. ^ Mo et al., 1997
  12. ^ Hardcastle et al., 1998
  13. ^ Motoyama et al., 1998
  14. ^ Yang et al., 1997
  15. ^ Park et al., 2000
  16. ^ Sheng et al., 2002
  17. ^ Oro and Higgins, 2003
  18. ^ Tojo et al., 2003b
  19. ^ Regl et al., 2004b
 
This article is licensed under the GNU Free Documentation License. It uses material from the Wikipedia article "Gli2". A list of authors is available in Wikipedia.
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