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GC Application Note
The analysis of oils, fat and fat contain- ing food containing food via fatty acid methyl esters (FAME) is a common task in governmental, quality control (QC) or contract research laboratories (CRO). Most often the samples are processed manually, whi
The BIOSTAT® Qplus with up to 12 culture vessels represents the basis for a professional and time-saving process optimization.
Design of experiments (DoE) is one of the most important techniques for systematic planning, execution and statistical evaluation of experiments. Although a DoE investigation is executable in one bioreactor, multi-bioreactor systems designed for pa
Thioflavin T aggregation assay to determine antibody stability using the FLUOstar Omega
The efficiency of antibody molecules is dependent on their stability. We introduce an assay setup where stability of antibodies is measured. Shaking stress is initiated by the FLUOstar Omega microplate reader and aggregation propensity of antibody ca
Comparison of growth in a test tube incubator to 96 well plates in a FLUOstar Omega with ACU
Growth of the fastidious microorganism Campylobacter jejuni was monitored using a FLUOstar Omega microplate reader equipped with the Atmospheric Control Unit (ACU) for independent control of oxygen and carbon dioxide. Growth was found tob e optimal a
Each antibody-antigen interaction has unique characteristics. A protocol giving good results for one antibody-antigen pair might found to be unsatisfactory for a second antibody even on the same sample. The interaction of antisera with protein epitop
Smart Solutions for large Molecules
As demonstrated here, there are a broad range of HPLC applications used in development, production, validation and release of protein therapeutics for human use. Some of the applications mentioned above are mandatory when submitting a new biopharmace
One of the most widely used water-soluble polymers is Polyvinylpyrrolidone (PVP). For more than 70 years it is applied in a variety of applications in medicine, pharmacy, cosmetics and industrial production. PVP binds to polar molecules exceptionally
High molecular weight polymers, bio molecules or particles in a range of a few nanometers up to several microns are more and more important in science and technology. Many applications are based on the specific properties of these materials. For this
The POLARstar Omega microplate reader from BMG LABTECH was used to study membrane fluidity via fluorescence polarization in 96-well format. The method allows for the monitoring of large scale alterations in membrane fluidity (such as phase transition
The detection of SNPs (single nucleotide polymorphisms) is an important tool for researchers in order to find mutations in DNA sequences. In this application note we describe a homogeneous fluorescence assay based on PCR. The Amplifluor® S