Ksep®50

The Ksep® 50 single-use continuous counterflow centrifugation system revolutionizes cell processing for advanced therapies by minimizing damaging shear forces that traditional centrifugation methods generate. This innovative approach preserves cell integrity while delivering exceptional recovery rates exceeding 90%, making it ideal for sensitive cell-based applications in biopharmaceutical manufacturing.

The automated system significantly reduces manual handling requirements, minimizing contamination risks throughout the process. With its closed-system design and single-use components, the Ksep® 50 ensures consistent, high-quality results while streamlining workflow efficiency. Cell processing times are reduced by 50% compared to conventional methods, enabling faster turnaround and increased productivity.

Designed for versatility, the system accommodates input volumes ranging from 0.1 L for process development to 20 L for commercial-scale production, facilitating seamless scale-up without method transfer complications. The technology demonstrates robust performance across an extensive range of cell densities, from very low concentrations below 1 million cells/mL to extremely high densities exceeding 100 million cells/mL.

The counterflow centrifugation principle applies gentle forces up to 2,000 g while maintaining cell viability with less than 5% change during processing. The 50 mL centrifuge chamber volume (2 × 25 mL) can process up to 10 billion cells per cycle, with peristaltic pump flow rates reaching 48 L/hr for efficient throughput.

Key applications include cell harvest, concentration, washing, media exchange, and clarification processes essential for cell therapy manufacturing. The system's sterile processing capabilities and single-use design eliminate cross-contamination concerns while reducing cleaning validation requirements, making it particularly valuable for GMP production environments.