Novagen and PerkinElmer Life Sciences Develop an Automated Solution for Protein Purification

05-Aug-2002
MADISON, WI and BOSTON, MA --- (August 1, 2002) --- Novagen, Inc., a leading provider of advanced reagents for molecular biology and proteomics research, and PerkinElmer Life Sciences, a leading provider of drug discovery, life science research and genetic disease screening solutions, today announced an agreement to co-market a novel new solution for high throughput, automated recombinant protein purification. “Extending our range of powerful liquid handling technologies to streamline the developing science of proteomics is a major goal for PerkinElmer Life Sciences,” said Warren Bock, vice president and general manager for liquid handling products. “We are first to market in this area because our systems are uniquely suited to integrate a broad range of products in all facets of drug discovery research.” “Novagen remains committed to offering only the highest quality reagents and kits, such as our RoboPop™ Purification Kits, which serve the emerging proteomics market,” said Dr. Robert Mierendorf, President of Novagen and Chief Technology Officer of CN Biosciences. “Our greatest satisfaction derives from providing tools that enable new discoveries.” The collaboration between PerkinElmer and Novagen has resulted in a breakthrough platform for automating labor-intensive steps involved in protein purification. Conventional purification methods are time consuming and labor intensive, requiring centrifugation and mechanical steps for cell harvest and lysis prior to purification. Using PerkinElmer’s MultiPROBE® II EX Robotic Liquid Handler and Novagen’s RoboPop™ protein purification Kits, the platform performs 96-well format purification of His•Tag® and GST•Tag™ fusion proteins directly from E. coli cultures without mechanical lysis or centrifugation. Novagen’s RoboPop System is uniquely suited for automated protein purification because it eliminates the need for centrifugation and enables the entire process from cell growth through purification to be performed in a single deep well plate. The kits feature PopCulture™ Reagent, rLysozyme™ Solution, and Benzonase® Nuclease for efficient extraction of intact proteins from total cultures, plus His•Mag™ or GST•Mag™ Agarose Beads and corresponding buffers for high capacity magnetic affinity purification. The MultiPROBE II EX system, when integrated with PerkinElmer’s Gripper™ Integration Platform, allows for complete walk-away automation of the magnetic affinity purification protocol. The system’s expanded deck configuration accommodates the RoboPop culture plate, reagents and Magnetight™ HT96™ Separation Stand. The RoboPop kits include everything needed to achieve the entire process, from cell culture of 96 recombinant clones to purified protein in an HTP 96-well format, including reagents and plasticware.

Other news from the department business & finance

Most read news

More news from our other portals

Fighting cancer: latest developments and advances

See the theme worlds for related content

Topic world Centrifugation

Centrifugation is an indispensable process in the life sciences. By using centrifugal forces, it enables the separation of cells, proteins and other biomolecules according to size and density. Whether in the purification of therapeutic proteins, the isolation of DNA/RNA or the separation of cell cultures - centrifugation plays a central role in biological and pharmaceutical research and production.

2 products
1 whitepaper
2 brochures
View topic world

Topic world Centrifugation

Centrifugation is an indispensable process in the life sciences. By using centrifugal forces, it enables the separation of cells, proteins and other biomolecules according to size and density. Whether in the purification of therapeutic proteins, the isolation of DNA/RNA or the separation of cell cultures - centrifugation plays a central role in biological and pharmaceutical research and production.

2 products
1 whitepaper
2 brochures