MADISON, WI and BOSTON, MA --- (August 1, 2002) --- Novagen, Inc., a leading provider of advanced
reagents for
molecular biology and
proteomics research, and
PerkinElmer Life Sciences, a leading provider of
drug discovery, life science research and genetic disease
screening solutions, today announced an agreement to co-market a novel new
solution for high throughput, automated recombinant protein
purification.
“Extending our range of powerful
liquid handling technologies to streamline the developing science of
proteomics is a major goal for
PerkinElmer Life Sciences,” said Warren Bock, vice president and general manager for liquid handling products. “We are first to market in this area because our systems are uniquely suited to integrate a broad range of products in all facets of drug discovery research.”
“Novagen remains committed to offering only the highest quality
reagents and
kits, such as our RoboPop™ Purification Kits, which serve the emerging proteomics market,” said Dr. Robert Mierendorf, President of Novagen and Chief Technology Officer of CN Biosciences. “Our greatest satisfaction derives from providing tools that enable new discoveries.”
The collaboration between PerkinElmer and Novagen has resulted in a breakthrough platform for automating labor-intensive steps involved in
protein purification. Conventional purification methods are time consuming and labor intensive, requiring
centrifugation and mechanical steps for
cell harvest and
lysis prior to purification. Using PerkinElmer’s MultiPROBE® II EX Robotic Liquid Handler and Novagen’s RoboPop™
protein purification Kits, the platform performs 96-well format purification of His•Tag® and GST•Tag™ fusion
proteins directly from E. coli cultures without mechanical lysis or centrifugation.
Novagen’s RoboPop System is uniquely suited for automated protein purification because it eliminates the need for centrifugation and enables the entire process from
cell growth through purification to be performed in a single deep well plate. The kits feature PopCulture™ Reagent, rLysozyme™ Solution, and Benzonase® Nuclease for efficient
extraction of intact
proteins from total cultures, plus His•Mag™ or GST•Mag™ Agarose Beads and corresponding
buffers for high capacity magnetic
affinity purification.
The MultiPROBE II EX system, when integrated with PerkinElmer’s Gripper™ Integration Platform, allows for complete walk-away
automation of the magnetic affinity purification protocol. The system’s expanded deck configuration accommodates the RoboPop culture plate, reagents and Magnetight™ HT96™ Separation Stand. The RoboPop kits include everything needed to achieve the entire process, from cell culture of 96 recombinant clones to purified protein in an HTP 96-well format, including reagents and plasticware.