22-Jul-2020 - Rice University

Better wastewater treatment? It's a wrap

Rice's trap-and-zap strategy for antibiotic resistant bugs becomes wrap, trap and zap

A shield of graphene helps particles destroy antibiotic-resistant bacteria and free-floating antibiotic resistance genes in wastewater treatment plants.

Think of the new strategy developed at Rice University as "wrap, trap and zap."

The labs of Rice environmental scientist Pedro Alvarez and Yalei Zhang, a professor of environmental engineering at Tongji University, Shanghai, introduced microspheres wrapped in graphene oxide in the Elsevier journal Water Research.

Alvarez and his partners in the Rice-based Nanosystems Engineering Research Center for Nanotechnology-Enabled Water Treatment (NEWT) have worked toward quenching antibiotic-resistant "superbugs" since first finding them in wastewater treatment plants in 2013.

"Superbugs are known to breed in wastewater treatment plants and release extracellular antibiotic resistance genes (ARGs) when they are killed as the effluent is disinfected," Alvarez said. "These ARGs are then discharged and may transform indigenous bacteria in the receiving environment, which become resistome reservoirs.

"Our innovation would minimize the discharge of extracellular ARGs, and thus mitigate dissemination of antibiotic resistance from wastewater treatment plants," he said.

The Rice lab showed its spheres -- cores of bismuth, oxygen and carbon wrapped with nitrogen-doped graphene oxide -- inactivated multidrug-resistant Escherichia coli bacteria and degraded plasmid-encoded antibiotic-resistant genes in secondary wastewater effluent.

The graphene-wrapped spheres kill nasties in effluent by producing three times the amount of reactive oxygen species (ROS) as compared to the spheres alone.

The spheres themselves are photocatalysts that produce ROS when exposed to light. Lab tests showed that wrapping the spheres minimized the ability of ROS scavengers to curtail their ability to disinfect the solution.

The researchers said nitrogen-doping the shells increases their ability to capture bacteria, giving the catalytic spheres more time to kill them. The enhanced particles then immediately capture and degrade the resistant genes released by the dead bacteria before they contaminate the effluent.

"Wrapping improved bacterial affinity for the microspheres through enhanced hydrophobic interaction between the bacterial surface and the shell," said co-lead author Pingfeng Yu, a postdoctoral research associate at Rice's Brown School of Engineering. "This mitigated ROS dilution and scavenging by background constituents and facilitated immediate capture and degradation of the released ARGs."

Because the wrapped spheres are large enough to be filtered out of the disinfected effluent, they can be reused, Yu said. Tests showed the photocatalytic activity of the spheres was relatively stable, with no significant decrease in activity after 10 cycles. That was significantly better than the cycle lifetime of the same spheres minus the wrap.

Facts, background information, dossiers
More about Rice University
  • News

    Cerium sidelines silver to make drug precursor

    Save your silver! It's better used for jewelry than as a catalyst for drugs. Rice University scientists have developed a greatly simplified method to make fluoroketones, precursors for drug design and manufacture that typically require a silver catalyst. Rice chemist Julian West and graduat ... more

    'Relaxed' T cells critical to immune response

    Like finding that needle in the haystack every time, your T cells manage what seems like an improbable task: quickly finding a few invaders among the many imposters in your body to trigger its immune response. T cells have to react fast and do so nearly perfectly to protect people from dise ... more

    Chemists show it's hip to be square

    Rice University chemists want to make a point: Nitrogen atoms are for squares. The nitrogens are the point. The squares are the frames that carry them. These molecules are called azetidines, and they can be used as building blocks in drug design. The Rice lab of chemist László Kürti introdu ... more