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Programmed cell death

Programmed cell-death (PCD) is death of a cell in any form, mediated by an intracellular program[1]. In contrast to necrosis, which is a form of cell-death that results from acute tissue injury and provokes an inflammatory response, PCD is carried out in a regulated process which generally confers advantage during an organism's life-cycle. PCD serves fundamental functions during both plant and metazoa (multicellular animals) tissue development.

Besides these two types of PCD, other pathways have been discovered.[3] Called "non-apoptotic programmed cell-death" (or "caspase-independent programmed cell-death" or "necrosis-like programmed cell-death") these alternative routes to death are as efficient as apoptosis and can function as either backup mechanisms or the main type of PCD.

Plant cells undergo particular processes of PCD which are similar to autophagic cell death. However, some common features of PCD are highly conserved in both plants and metazoa.

The concept of "programmed cell-death" was used by Lockshin & Williams [4]in 1964 in relation to insect tissue development, around eight years before "apoptosis" was coined. Since then, PCD has become the more general of these two terms.

PCD has been the subject of increasing attention and research efforts. This trend has been highlighted with the award of the 2002 Nobel Prize in Physiology or Medicine to Sydney Brenner (United Kingdom), H. Robert Horvitz (US) and John E. Sulston (UK). [5]


Programmed cell-death in plant tissue

In "APL regulates vascular tissue identity in Arabidopsis", [6] Bonke and colleagues state that one of the two long-distance transport systems in vascular plants, xylem, consists of several cell-types "the differentiation of which involves deposition of elaborate cell-wall thickenings and programmed cell-death." The authors emphasize that the products of plant PCD play an important structural role.

Basic morphological and biochemical features of PCD have been conserved in both plant and animal kingdoms [7]. It should be noted, however, that specific types of plant cells carry out unique cell-death programs. These have common features with animal apoptosis -- for instance, nuclear DNA degradation -- but they also have their own peculiarities, such as nuclear degradation being triggered by the collapse of the vacuole in tracheary elements of the xylem. [8]

Janneke Balk and Christopher J. Leaver, of the Department of Plant Sciences, University of Oxford, carried out research on mutations in the mitochondrial genome of sun-flower cells. Results of this research suggest that mitochondria play the same key role in vascular plant PCD as in other eukaryotic cells. [9]

PCD in pollen prevents inbreeding

During pollination, plants enforce self-incompatibility (SI) as an important means to prevent self-fertilization. Research on the corn poppy (Papaver rhoeas) has revealed that proteins in the pistil on which the pollen lands, interact with pollen and trigger PCD in incompatible (ie. self) pollen. The researchers, Steven G. Thomas and Veronica E. Franklin-Tong, also found that the response involves rapid inhibition of pollen-tube growth, followed by PCD. [10]

Programmed cell death in slime molds

The social slime mold Dictyostelium discoideum has the peculiarity of adopting either a predatory amoeba-like behavior in its unicellular form, or coalescing into a mobile slug-like form when dispersing the spores which will give birth to the next generation.[11]

The stalk is composed of dead cells which have undergone a type of PCD that shares many features of an autophagic cell-death: massive vacuoles forming inside cells, a degree of chromatin condensation, but no DNA-fragmentation. [12] The structural role of the residues left by the dead cells is reminiscent of the products of PCD in plant tissue.

D. discoideum is a slime mold, part of a branch which may have emerged from eukaryotic ancestors about a billion years before the present. They apparently emerged after the ancestors of green-plants and the ancestors of fungi and animals had differentiated. But in addition to their place in the evolutionary tree, the fact that PCD has been observed in the humble, simple, six-chromosome D. discoideum has additional significance: it permits the study of a developmental PCD path which does not depend on the caspases which are characteristic of apoptosis. [13]

Evolutionary origin of PCD

Biologists had long suspected that mitochondria originated from bacteria which had been incorporated as endosymbionts ("living together inside") of larger eukaryotic cells. It was Lynn Margulis who from 1967 on championed this theory, which has since become widely accepted. [14] The most convincing evidence for this theory is the fact that mitochondria possess their own DNA and are equipped with genes and replication apparatus.

This evolutionary step would have been more than risky for the primitive eukaryotic cells which began to engulf the energy-producing bacteria and conversely, a perilous step for the ancestors of mitochondria which began to invade their proto-eukaryotic hosts. This process is still evident today, between human white blood-cells and bacteria. Most of the time, invading bacteria are destroyed by the white blood-cells; however, it is not uncommon for the chemical warfare waged by prokaryotes to succeed, with the consequence known as infection and by its resulting damage.

One of these rare evolutionary events, about two billion years before the present, made it possible for certain eukaryotes and energy-producing prokaryotes not only to coexist, but to mutually benefit from their symbiosis. [15]

Mitochondriate eukaryotic cells live poised between life and death, because mitochondria still retain their repertoire of molecules which can trigger cell suicide. [16] This process has now been evolved to happen only when programmed. Given certain signals to cells (such as feedback from neighbors, stress or DNA-damage), mitochondria release caspase activators which trigger the cell-death inducing biochemical cascade. As such, the cell-suicide mechanism is now crucial to all of our lives.

Programmed death of entire organisms

Main article: Phenoptosis


  1. ^ Engelberg-Kulka H, Amitai S, Kolodkin-Gal I, Hazan R (2006). "Bacterial Programmed Cell Death and Multicellular Behavior in Bacteria". PLoS Genetics 2 (10).
  2. ^ Lawrence M. Schwartz et al. (1993). "Do All Programmed Cell Deaths Occur Via Apoptosis?". PNAS 90 (3): 980.;and, for a more recent view, see W. Bursch et al. (2000). "Programmed Cell Death (PCD): Apoptosis, Autophagic PCD, or Others?". Annals of the New York Academy of Sciences 926: 1.
  3. ^ Kroemer G, Martin SJ (2005). "Caspase-independent cell death". Nat Med. 11 (7): 725-30.
  4. ^ Richard A. Lockshin, Carroll M. Williams (1964). "Programmed cell death—II. Endocrine potentiation of the breakdown of the intersegmental muscles of silkmoths". Journal of Insect Physiology 10 (4): 643-649.
  5. ^ 2002 Nobel Prize in Medicine or Physiology[1]
  6. ^ Martin Bonke et al., published in Nature Vol. 425, Nov. 13, 2003, p. 181.
  7. ^ Mazal Solomon, et al.: "The Involvement of Cysteine Proteases and Protease Inhibitor Genes in the Regulation of Programmed Cell Death in Plants", The Plant Cell, Vol. 11, 431-444, March 1999. See also related articles in The Plant Cell Online, [2]
  8. ^ See Jun Ito and Hiroo Fukuda: "ZEN1 Is a Key Enzyme in the Degradation of Nuclear DNA during Programmed Cell Death of Tracheary Elements", The Plant Cell, Vol. 14, 3201-3211, December 2002.
  9. ^ "The PET1-CMS Mitochondrial Mutation in Sunflower Is Associated with Premature Programmed Cell Death and Cytochrome c Release", The Plant Cell, Vol. 13, 1803-1818, August 2001
  10. ^ Thomas, and Franklin-Tong: "Self-incompatibility triggers programmed cell death in Papaver pollen", Nature Vol. 429, 20 May 2004, p. 305.
  11. ^ Crespi B, Springer S: "Ecology. Social slime molds meet their match" Science 2003 Jan 3;299(5603):105-6 [3].
  12. ^ See the article by Levraud et al.: "Dictyostelium cell death : early emergence and demise of highly polarized paddle cells", The Journal of Cell Biology Vol. 160, 7, p. 1105 [4].
  13. ^ See also Roisin-Bouffay et al.: "Developmental Cell Death in Dictyostelium Does Not Require Paracaspase", The Journal of Biological Chemistry Vol. 279, 12, p. 11489, 19 March 2004 [5].
  14. ^ see "The Birth of Complex Cells", by Christian de Duve, Scientific American Vol. 274, 4, April, 1996
  15. ^ See "Ancient Invasions: From Endosymbionts to Organelle", by Sabrina D. Dyall et al., Science Vol. 304 p. 253, 9 April. 2004[6].
  16. ^ see Chiarugi and Moskowitz, in Science 297, p. 200[7]

See also

This article is licensed under the GNU Free Documentation License. It uses material from the Wikipedia article "Programmed_cell_death". A list of authors is available in Wikipedia.
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