MolYsis: Selective Enrichment and Isolation of Bacterial and Fungal DNA from Host Material
New: Molzym’s MolYsis technology allows now for selective enrichment and isolation of bacterial and fungal DNA from 0.2-10 ml liquid samples and tissues more
my.bionity.com
With an accout for my.bionity.com you can always see everything at a glance – and you can configure your own website and individual newsletter.
SepsiTest™-UMD CE IVD allows fast molecular detection and identification of bacteria and fungi. The test is independent of whether pathogens can grow or are inhibited by antibiotics.
The removal of human DNA and free-circulating microbial DNA ensures high detection specificity of exclusively DNA of intact cells. In clinical evaluations, pathogens of more than 200 bacterial and 65 fungal genera have been identified so far. SepsiTest-UMD CE IVD uses broad-range 16S and 18S rRNA gene PCR/Real-Time PCR assays with subsequent sequence analysis. Thus the test is eu-bacterial and pan-fungal.
Application
SepsiTest-UMD CE IVD is particularly suited for the diagnosis of pathogens in samples from patients who are on antibiotic therapy, regarding, for example, the following diseases:
|
|
|
|
|
|
|
|
Handling/Laboratory Equipment
For the implementation of SepsiTest-UMD CE IVD only standard equipment of a molecular biology laboratory is required.
SepsiTest-UMD CE IVD includes all reagents and consumables necessary for DNA extraction and broad range PCR or Real-Time PCR analysis as well as primers for Sanger-sequencing.
The workflow comprises of the following steps:
Specimens
Various specimens can be analyzed by SepsiTest-UMD CE IVD in the diagnostic routine:
New: Add-On 10 CE IVD kit for diagnosis of up to 10ml fluid samples used with SepsiTest-UMD CE IVD.
Sensitivity
Based on spiking experiments using 1ml EDTA blood, the analytical sensitivities of various pathogens were tested, among them S. aureus (limit of detection: 20 cfu/ml), E. coli (40 cfu/ml) and C. albicans (10 cfu/ml).
Automation
Automation reduces hands-on time, costs and contamination risk. Molzym offers diagnostic systems that include semi-automated (UMD-SelectNA™ CE IVD) and fully-automated DNA extraction (Micro-Dx™).
For more detailed information, including a list of publications, a quotation or presentation in your laboratory, please feel free to contact the Molzym support-team.
MolYsis: Selective Enrichment and Isolation of Bacterial and Fungal DNA from Host Material
New: Molzym’s MolYsis technology allows now for selective enrichment and isolation of bacterial and fungal DNA from 0.2-10 ml liquid samples and tissues more
New: Ultra-Sensitive PCR Reagents for Molecular Microbiology
Molzym offers a range of ultra-clean PCR products for 16S PCR. All kits are free of contaminating DNA enabling ultra-sensitive and reliable PCR-results more
Unique Automated Pathogen DNA Extraction and Broad-Range Molecular Diagnosis of Bacteria and Fungi
Culture-independent automated pathogen enrichment and DNA extraction combined with broad-range PCR-based species detection and identification more
Molzym is Part of Gradian Diagnostics
Gradian Diagnostics announced that Molzym GmbH & Co. KG is joining Gradian Diagnostics. Molzym will become the first acquisition, bringing a wide set of products and services due to their wealth of experience and expertise in molecular microbial analysis, and their focus on deep research an ... more
Molzym Automated Pathogen Enrichment and PCR Test Gets CE Mark for In Vitro Diagnostics
Molzym announces CE IVD marking of their robotic microbial DNA isolation and direct PCR test, Micro-Dx™, for routine pathogen diagnosis. With Micro-Dx™ Molzym introduces its latest development of products in line with SepsiTest™-UMD which was released in 2008. “By Molzym’s proprietary robot ... more
Seegene and Molzym partner on automated molecular sepsis diagnostics
Seegene and Molzym announced a partnership on the automated Magicplex™ Sepsis, a new multi-pathogen screening test capable of quick and accurate identification of over 90 leading sepsis-causing pathogens. "The Magicplex Sepsis Test will be the new gold standard for performing accurate, rapi ... more
Direct Eubacterial PCR Diagnosis of Primary Sterile Body Fluids and Tissues
Culture-inde¬pend¬ent molecular identification is a straight forward approach for the rapid pathogen identification, including fastidious and antibiotic-inhibited microorganisms. CE-IVD UMD-Universal has been evaluated using primary sterile body flui more
Company Molzym was founded as a privately owned enterprise in Bremen, North-West Germany, in 2003. Ever since its foundation, Molzym was committed to develop innovative solutions to serve biological research and diagnosis of infectious diseases. Products range from pre-analytical kits for ... more