01-06-2011

Abstract The RealTime ready Configurator is a web-based configuration and ordering portal for function tested, custom RT-qPCR assays based on Universal ProbeLibrary (UPL) technology. It contains consolidated gene annotation information derived from three key public gene annotation resources ...

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01-06-2011

Abstract On the RealTime ready Configurator [1], we offer function tested human, mouse, and rat qPCR assays for gene expression quantification based on the unique Universal ProbeLibrary technology (UPL) [2]. The Universal ProbeLibrary is a set of short hydrolysis probes (8–9-mers) containing ...

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16-06-2010

Bioscience Genotyping and Mapping Division, The Australian National University SNPs were easily detected with the Amplifluor® System from Millipore Detailed protocol and instrument settings are shown for this assay BMG LABTECH’s FLUOstar OPTIMA has proved to be an excellent reader for this ...

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25-01-2010

Introduction Although congenital heart malformations are the most common birth defects in humans, the underlying patho­mechanisms remain widely unknown. Through linkage analyzes and candidate-gene approaches, several gene mutations causing congenital heart defects (e.g., CITED2, GATA4, ...

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04-01-2010

Pyrosequencing of complementary DNA-PCR ampli­cons was used to determine comprehensive major histocompatibility complex (MHC) class-I genotypes in primates that provide essential preclinical models for studies of infectious disease, vaccine development, and transplantation. By sequence-based ...

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21-12-2009

Introduction High Resolution Melting (HRM) is a novel and promising screening technique used for mutation analysis and detection. Its application prevents the need to sequence all the exons of a gene when looking for novel mutations. The method is therefore time- and cost-effective for the ...

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16-12-2009

Introduction The novel small molecule inhibitors are often substrates of ABC transporters [1] which influence the pharmacokinetic parameters of the drug candidates and cause drug resistance of tumor cells [2,3]. Therefore expression profiling of ABC transporter genes in cell lines used for ...

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14-12-2009

Introduction Neuroblastoma cell lines can be models for the responsiveness of neuroblastomas to chemotherapy-induced cell death as well as for the sensitivity of untransformed neuro-nal cells to toxic side-effects of chemotherapy. Both phenom-ena are relevant to neuroblastoma cells exposed ...

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A Cross-Platform Comparison

09-12-2009

Introduction During the past few years real-time PCR has been proven to be a key technology for gene expression analysis, for instance for gene expression profiling and array verification studies and for the genotyping of single nucleotide polymorphisms, both in industrial and public ...

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07-09-2009

Introduction The Smart Amplification process, version 2 (SMAP-2), also known as SmartAmp-2, is a rapid polymorphism detection system based on a unique asymmetric primer design, including a folding primer (FP) and a turn-back primer (TP) [1]. Combined with Thermus aquaticus MutS (Taq MutS) ...

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