Ross VerHeul, Shawn Sternisha, Emilie Bouda, Shaofeng Wang, Akash Bhattacharya

Adapting the Power of Density Gradient Separations for Gene Therapy Analytics

DGE-AUC (Density Gradient Equilibrium AUC) highly simplified analytical method

Density gradient ultracentrifugation (DGUC) has long been considered a staple technology providing high-resolution purification of numerous materials in gene therapy, including AAV, adenovirus, and plasmids, from bench to manufacturing scales. With DGE-AUC you can monitor and quantify this density gradient process to get the simple, intuitive nature of DGUC (Gold standard for purification) in an analytical instrument (Optima AUC) with a high resolution, high senssitive, and serotype independent method.

DGE-AUC advantages:

  • More Data (DGE AUC is orthogonal to well established SV AUC methods)
  • Less Sample (more than 30X greater sensitivity is achievable)
  • Higher Throughput (at least 3x samples; e.g., 6 sector CPs)
  • Higher Resolution (not time resolved; no wavelength/resolution trade off; scan averaging)
  • More Straightforward (rapid, intuitive data interpretation with Excel)
  • More Sample Types (serotype agnostic; not size limited; e.g., AAV, adeno, plasmids)

Facts, background information, dossiers
  • AAV
  • density gradient ul…
  • orthogonal technique
  • serotype agnostic
  • virus characterization
  • virus purification
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