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Multiplexed optogenetic control, using PA- Vav2 and PI-Rac1 in the same cell

Multiplexed optogenetic control, using Photo-inhibitable Vav2 (PA-Vav2) and Photo-inhibitable Rac1 (PI-Rac1) in the same cell.

Upon irradiation, area and roundness changed as they did in response to PA-Vav2 alone, but edge dynamics were clearly reduced, unlike response to either PA-Vav2 alone or PI-Rac1 alone. Blue dot denotes irradiation. Cells were visualized using mVenus-PI-Rac1.
By Onur Dagliyan. Hahn Lab copyright 2016.

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