Assay Design, Validation and Analysis - Primers and Probes for qPCR and dPCR Applications

Probe-based qPCR or digital PCR relies on sequence-specific detection of a desired PCR product. Unlike dye-based methods that detect all double-stranded DNA, probe-based approaches use a fluorescently labeled target-specific probe, resulting in increased specificity and sensitivity. You have the option to adjust binding affinity via MGB, LNA, and other Tm enhancers, or even combine different Tm enhancers to tailor your assay to your individual needs. By using well-designed, high-quality probes in your analysis, you reduce the cost of assay optimization, potential error correction, and replicates of your measurements. Alternatively, the development, validation, manufacturing and testing of qPCR and digital PCR assays can be outsourced to Microsynth, your partner with more than 20 years of experience in the design and development of digital PCR and qPCR assays for various applications.