UV/VIS Spectrophotometry has become a standard method used on a daily basis in many life science laboratories. This is mainly due to its simplicity; it does not require complex sample preparation, it is easy to perform and results are obtained within seconds.
A typical measurement requires only a small amount of sample, and as it is a non-destructive method, samples can be used for subsequent analyses. The field of life sciences typically applies UV/VIS spectrophotometry in the analysis of nucleic acids, proteins and bacterial cell cultures.
Our UV/VIS Life Science Applications in a Nutshell guide deepens into the most common applications:
- Concentration determination of nucleic acids – DNA and RNA.
- Purity of nucleic acids.
- Concentration determination of proteins by direct measurement or colorimetric assays, study of enzymatic reactions, and monitoring growth curves of bacterial cell suspensions.