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16 Current white paper of BMG Labtech
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Growth of Campylobacter using BMG LABTECH’s FLUOstar Omega equipped with ACU
Comparison of growth in a test tube incubator to 96 well plates in a FLUOstar Omega with ACU
03-11-2011
Growth of Microaerophiles using Campylobacter as a model Comparison of growth in a traditional environmentally controlled test tube incubator to 96 well plates in a FLUOstar Omega with ACU Independent Oxygen and Carbon Dioxide regulation allows for optimal growth conditions for different ...
Promotion of Aggregation as a Means of Assessing the Stability of Antibody Molecules
Thioflavin T aggregation assay to determine antibody stability using the FLUOstar Omega
03-11-2011
Stress can cause denaturation and aggregation of antibodies Aggregation propensity of antibody candidates was measured using a fluorescence based assay in 96-well format Shaking stress initiated by the FLUOstar Omega and the addition of Teflon beads enabled a successful antibody ...
Miniaturised Platelet Aggregation Assays using the NOVOstar Microplate Reader
25-05-2011
A new platelet aggregation method is introduced using the NOVOstar microplate reader Platelets aggregation in presence of agonists was supported by different shaking settings Data is in accordance with values obtained using conventional low-throughput methods Introduction Platelets are ...
Assessing Microbial Metabolism Using A Simple ‘Mix & Measure’ Oxygen Consumption Assay
25-05-2011
High throughput analysis of microbial O2 consumption to assess microbial metabolism MitoExpress®, water soluble “mix and measure” O2 sensing probes Multiplexing with FLUOstar Omega to measure both growth (OD 600) and O2 consumption Introduction MitoExpress® (Luxcell Biosciences) is a ...
Membrane Fluidity Measurements Using UV Fluorescence Polarization and the POLARstar Omega
23-06-2010
Membrane fluidity is measured in both liposomes and mitochondrial membranes via fluorescence polarization UV FP optics are used on the POLARstar Omega to measure DPH (1,6-diphenyl-hexa-1,3,5-triene) fluorescence The effects of dibucaine and propranolol on membrane fluidity are ...
alamarBlue® Assay for Assessment of Cell Proliferation using the FLUOstar OPTIMA
23-06-2010
alamarBlue® cell proliferation assay is based on a reducing environment that indicates metabolic active cells Cell proliferation on different substrates was followed over 17 days with the FLUOstar OPTIMA Fluorescent and colorimetric detection possible Introduction A variety of methods have ...
Application of the Amplifluor® SNPs Genotyping System using a FLUOstar OPTIMA microplate reader
16-06-2010
Bioscience Genotyping and Mapping Division, The Australian National University SNPs were easily detected with the Amplifluor® System from Millipore Detailed protocol and instrument settings are shown for this assay BMG LABTECH’s FLUOstar OPTIMA has proved to be an excellent reader for this ...
Enzyme Kinetic Measurements for a Combinatorial Library of Inhibitors of Pseudomonas Elastase
25-11-2009
Pseudomonas elastase activity monitored using Abz-Nba internally quenched substrate Km and Vmax calculated using MARS Data Analysis Software Michaelis Menten kinetics determined for a library of 160 elastase inhibitors Introduction Pseudomonas elastase (pseudolysin, LasB) is a ...
ORAC Assay Performed on the POLARstar Omega and PHERAstar FS microplate reader
15-09-2009
Antioxidants are able to neutralize Reactive Oxygen Species Antioxidant capacity of fruit juices determined with the ORAC assay in 96 and 384-well format MARS data analysis software with predefined templates for quick ORAC evaluation Introduction In all oxygen consuming cells, metabolism ...
Analysis of Prostate Tumour Cell Invasion Using BD FluoroBlokTMand FLUOstar OPTIMA
Real-time analysis of tumour cell invasion with a FLUOstar OPTIMA enclosed in an AtmosBag
24-06-2009
Introduction The mechanism of prostate metastasis to bone marrow is a complex multistage process that is only beginning to be understood. Initial steps include down regulation of molecular binding complexes and production of enzymes such as matrix metalloproteinases (1) leading to the loss of ...
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