My watch list
my.bionity.com  
Login  

Predicting the Long-Term Stability of Biologics

Analysis of Formulation-Dependent Colloidal and Conformational Stability of Monoclonal Antibodies

Franziska Söltl, Jonathan Derix, Patrick Garidel, Michaela Blech, Dennis Breitsprecher

Relation between conformational and colloidal stability of antibodies

Schematic representation of the backreflection principle to detect protein aggregation

Conformational stability and aggregation of a mAb under different buffer conditions

The growing number of biological drugs such as monoclonal antibodies (mAbs), as well as the wealth of heterogeneity between mAb variants requires a thorough development process to maximize mAbs compliance with regulation. Therefore, biophysical analytical methods are required already at early stages of the development process to guide and streamline further antibody processing and to predict antibody developability. In this case study, we demonstrate how the Prometheus NT.48 can be used to predict long-term mAb stability in a formulation screen by simultaneous quantification of both, conformational and colloidal stability of biologicals in thermal gradients.

Facts, background information, dossiers
  • long-term stability
  • conformational stability
  • colloidal stability
  • biologicals
  • aggregation
  • backreflection
  • protein aggregation
  • protein aggregate c…
More about NanoTemper Technologies
  • White papers

    Assess Enzyme Stability without Buffer Limitations

    Would you like to assess enzyme stability without any buffer restrictions? Read more about thermal unfolding analysis of an esterase in detergents more

    nanoDSF Goes GPCR Characterization

    Learn how nanoDSF evaluates compounds for lead discovery and improves receptor stability for biophysical assays by screening buffer conditions more

    Predicting the Long-Term Stability of Biologics

    Learn how to predict long-term mAb stability in a formulation screen by quantification of conformational and colloidal stability more

  • Videos

    A Revolution in Protein Stability Analysis

    The assessment of thermal stability parameters of biologics is an integral part of biopharmaceutical research. The ever growing number of biologics in the development pipelines worldwide demands rapid and precise methods to quickly screen large sets of conditions in an easy and straightforw ... more

  • News

    Growth Champions 2017

    We have some great news! FOCUS and Statista have ranked the 500 fastest-growing companies in Germany and we made the list! They identified companies with exceptional growth rates between the years 2012 and 2015 as “Growth Champions 2017”. We’re proud to announce that NanoTemper Technologies ... more

  • Companies

    NanoTemper Technologies GmbH

    NanoTemper Technologies is deeply committed to the best customer experience. Central to this is a strong focus on enabling researchers to easily, efficiently, and accurately perform protein characterization. With a broad offering of systems, software and consumables for evaluating binding a ... more

Your browser is not current. Microsoft Internet Explorer 6.0 does not support some functions on Chemie.DE