Now ready - Easy Bispecific Antibody Evaluation

FortéBio - A Sartorius Brand

Analyze specific binding of bsAbs in addition to IgG titer in a single high-throughput assay

The production of bispecific antibodies adds a challenge for scientists in the cell line screening process since many other conformations of antibodies can be produced by the cells. If screening is based only on Protein A titer, good pools and clone candidates might be discarded. For this reason, a high-throughput assay using the Octet® HTX system to identify pools and clones with higher concentrations of bispecific antibodies was developed.

This novel BLI approach offers an easy screening method and workflow that assesses bsAb interactions in a versatile, label-free, and easy-to-use format.

There is currently a limitation in technologies that allow for quantitative functional assessment of two interactions to one bispecific molecule in the market. The AppNote shows an easy method for bsAb evaluation. Utilizing Protein A and HIS1K biosensors to identify conditions quickly that are suitable for quantitative measurement of bispecific binding.

By analyzing specific binding results in addition to IgG titer in a single high-throughput instru-ment, the binding activity of a bispecific molecule to both targets can be assessed to rank the top pools/clones during cell line development. The results obtained from the Octet binding assay shown have been validated using other analytical methods such as RP-HPLC, CE-SDS, SPR, and LC-MS to show that binding results can be correlated to the purity of the bsAb from the samples tested.

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Applications

antibody screening
cell line screening
concentration measurement

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Now ready - Easy Bispecific Antibody Evaluation

Analyze specific binding of bsAbs in addition to IgG titer in a single high-throughput assay

FortéBio - A Sartorius Brand

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Topic world Antibodies

Antibodies are specialized molecules of our immune system that can specifically recognize and neutralize pathogens or foreign substances. Antibody research in biotech and pharma has recognized this natural defense potential and is working intensively to make it therapeutically useful. From monoclonal antibodies used against cancer or autoimmune diseases to antibody-drug conjugates that specifically transport drugs to disease cells - the possibilities are enormous

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Topic world Antibodies

Antibodies are specialized molecules of our immune system that can specifically recognize and neutralize pathogens or foreign substances. Antibody research in biotech and pharma has recognized this natural defense potential and is working intensively to make it therapeutically useful. From monoclonal antibodies used against cancer or autoimmune diseases to antibody-drug conjugates that specifically transport drugs to disease cells - the possibilities are enormous