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Contamination-Free and Precise Pipetting of Blood and Plasma

Reliable exclusion of sample carry-over and cross-contamination during pipetting of blood and plasma with positive displacement pipettes

NADÈGE BELHADJ, Gilson SAS

Contamination-free pipetting of plasma with a direct displacement pipette (Microman® E).

Two pipetting concepts: Air displacement cushion pipette (left) and positive displacement pipette (right). Positive displacement pipettes have no air cushion, so the accuracy of the pipette is not affected by the physical properties of the sample (e.g. viscosity and temperature).

Positive-displacement pipettes allow precise pipetting of blood without foam formation

Systematic and random error in the pipetting of blood with MICROMAN® E compared to the standard pipette (with air cushion). The measurements were based on the average of ten gravimetric measurements per sample

Blood and plasma contain proteins that can lead to foaming during pipetting. This makes it difficult to pipette blood and plasma accurately and contamination-free. Blood is also a vis-cous suspension and may be dangerous. With a positive displacement pipette, the risk of contamination from the sample to the pipette and user is reduced and the absence of the air cushion does not affect the accuracy of the pipette due to the physical properties of the sample (e.g. viscosity and temperature). Disposable Capillary Pistons are the only way to eliminate residual sample carry-over, aero-sol contamination, and cross-contamination when pipetting liquids like blood or plasma. With the disposable piston located inside the capillary tip, your pipette, your sample and yourself are protected against contamination.

 

This white paper shows how blood and plasma can be pipetted accurately and without con-tamination by comparing positive displacement and air displacement pipetting.

The following questions will be answered:

  • What problems often occur when pipetting plasma and blood?
  • How does a positive displacement pipette work?
  • What are the advantages of the direct displacement pipette?

Facts, background information, dossiers
  • blood
  • positive-displaceme…
  • air-displacement pipettes
More about Gilson
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