Culture-Independent Diagnosis of Bacterial and Fungal DNA in Blood, Other Body Fluids and Tissues
Culture-independent, CE IVD-marked diagnosis of pathogens of 200 bacterial and 65 fungal genera in tissue biopsies, blood and other body fluids more
my.bionity.com
With an accout for my.bionity.com you can always see everything at a glance – and you can configure your own website and individual newsletter.
Host DNA negatively influences the sensitivity and specificity of molecular analysis of microorganisms in human and animal samples.
The groundbreaking MolYsis technology provides a solution for the removal of non-target host DNA and enrichment of intact microorganisms. Applications comprise next generation sequencing, amplicon cloning, real-time PCR, Sanger sequencing and DNA array analysis of microorganisms.
Protocols are available that allow the MolYsis pre-treatment of 0.2-10ml samples followed by microbial DNA extraction and purification. The MolYsis Complete kits include the entire process of MolYsis pre-treatment and microbial DNA purification.
The MolYsis Basic kits include the removal of host DNA and enrichment of microbial DNA and can be combined with any other commercial DNA purification kit. A great variety of liquid samples can be processed, among them whole blood, CSF, BAL, synovial fluid, pleural fluid, urine and others.
Tissue biopsies and other materials as well as liquid samples can be extracted using the Ultra-Deep Microbiome Prep kits.
A walk-away automated version of the MolYsis technology, MolYsis-SelectNA plus, is available as well. All kit components are guaranteed free of contaminating microbial DNA.
The Process of Selective Isolation of Bacterial and Fungal DNA
The MolYsis technology includes a process by which non-target DNA is liberated from the host cells by a selective lysis of human or animal cells. In the following extraction steps, the host DNA is digested by a DNase treatment, a reagent lyses a broad variety of Gram-positive and Gram-negative bacteria and fungi. Further, PCR inhibitors are removed, finally providing pure, enriched microbial DNA.
MolYsis has been validated with more than 200 genera of bacteria (86 Gram-positives, 120 Gram-negatives) and 65 genera of fungi from the clinical routine.
Key Benefits:
Optimal detection of microbial DNA in clinical specimens is achieved by the combination of MolYsis extraction with Molzym‘s highly active, DNA-free MolTaq 16S/18S polymerase or with the ready-to-use DNA-free Mastermix products.
Culture-Independent Diagnosis of Bacterial and Fungal DNA in Blood, Other Body Fluids and Tissues
Culture-independent, CE IVD-marked diagnosis of pathogens of 200 bacterial and 65 fungal genera in tissue biopsies, blood and other body fluids more
Unique Automated Pathogen DNA Extraction and Broad-Range Molecular Diagnosis of Bacteria and Fungi
Culture-independent automated pathogen enrichment and DNA extraction combined with broad-range PCR-based species detection and identification more
DNA-free PCR Reagents for Sensitive Analyses in Molecular Microbiology
Best suited for standard and Real-Time PCR ✓ Up to 40 amplification cycles without background ✓ Ultra-sensitive detection of bacterial and fungal DNA ✓ more
Molzym Announces Double-Digit Million EUR Capital Increase
Molzym is proud to announce a double-digit million EUR investment by a large single family office. The capital will be deployed for the international business expansion, in particular for market entry and expansion in the US and China as well as further expanding its presence in Europe. Fur ... more
Molzym is Part of Gradian Diagnostics
Gradian Diagnostics announced that Molzym GmbH & Co. KG is joining Gradian Diagnostics. Molzym will become the first acquisition, bringing a wide set of products and services due to their wealth of experience and expertise in molecular microbial analysis, and their focus on deep research an ... more
Molzym Automated Pathogen Enrichment and PCR Test Gets CE Mark for In Vitro Diagnostics
Molzym announces CE IVD marking of their robotic microbial DNA isolation and direct PCR test, Micro-Dx™, for routine pathogen diagnosis. With Micro-Dx™ Molzym introduces its latest development of products in line with SepsiTest™-UMD which was released in 2008. “By Molzym’s proprietary robot ... more
Direct Eubacterial PCR Diagnosis of Primary Sterile Body Fluids and Tissues
Culture-inde¬pend¬ent molecular identification is a straight forward approach for the rapid pathogen identification, including fastidious and antibiotic-inhibited microorganisms. CE-IVD UMD-Universal has been evaluated using primary sterile body flui more
Company Molzym was founded as a privately owned enterprise in Bremen, North-West Germany, in 2003. Ever since its foundation, Molzym was committed to develop innovative solutions to serve biological research and diagnosis of infectious diseases. Products range from pre-analytical kits for ... more