Single Cell and Tissue Metabolic Phenotyping

The QSM™ platform from Doppelganger enables, for the first time, high-resolution and precise analysis of cellular bioenergetics—directly from tissues, including formalin-fixed paraffin-embedded (FFPE) samples, at single-cell resolution, and through the quantitative measurement of substrate uptake in both living and fixed cells. This marks a new chapter in functional cell biology, extending far beyond the limitations of conventional Seahorse-based analyses.

A key advantage of this technology lies in its ability to measure cellular energy production directly from tissue samples. By incorporating FFPE material, valuable biobank collections and clinical specimens can be analyzed retrospectively. This unique capability allows researchers to correlate energetic markers with histopathological findings, opening new diagnostic and therapeutic opportunities.

QSM™ also provides the first-ever capability to profile cellular respiration and ATP production at single-cell resolution. This high-definition perspective reveals metabolic heterogeneity within tissues and uncovers rare but biologically significant cell populations. For drug discovery, this means disease mechanisms can be understood with greater precision and therapies can be developed with enhanced specificity.

Another distinguishing feature is the quantitative analysis of substrate uptake. QSM™ makes it possible to determine how cells utilize different nutrients such as glucose or fatty acids, and how these metabolic fluxes shift under disease conditions or therapeutic interventions. This generates a robust foundation for assessing the effects of candidate drugs on cellular metabolism and for identifying novel therapeutic targets.

By uniting functional analysis with spatial resolution, the QSM™ platform offers researchers and developers unparalleled insights into the dynamics of cellular metabolism. At the same time, it provides a strong basis for the discovery of novel cellular mechanisms, the validation of targeted therapeutics, and the generation of clinically relevant, publication-ready findings.

QSM™ – Your key to unlocking cellular bioenergetics.