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LineweaverBurk plotIn biochemistry, the LineweaverBurk plot (or double reciprocal plot) is a graphical representation of the LineweaverBurk equation of enzyme kinetics, described by Hans Lineweaver and Dean Burk in 1934^{[1]}. Additional recommended knowledge
DerivationThe plot provides a useful graphical method for analysis of the MichaelisMenten equation: Taking the reciprocal gives where V is the reaction velocity, K_{m} is the MichaelisMenten constant, V_{max} is the maximum reaction velocity, and [S] is the substrate concentration. UseThe LineweaverBurk plot was widely used to determine important terms in enzyme kinetics, such as K_{m} and V_{max} before the wide availability of powerful computers and nonlinear regression software, as the yintercept of such a graph is equivalent to the inverse of V_{max}; the xintercept of the graph represents 1/K_{m}. It also gives a quick, visual impression of the different forms of enzyme inhibition. The double reciprocal plot distorts the error structure of the data, and it is therefore unreliable for the determination of enzyme kinetic parameters. Although it is still used for representation of kinetic data^{[2]}, nonlinear regression or alternative linear forms of the MichaelisMenten equation such as the EadieHofstee plot are generally used for the calculation of parameters^{[3]}. When used for determining the type of enzyme inhibition, the LineweaverBurk plot can distinguish competitive, noncompetitive and uncompetitive inhibitors. Competitive inhibitors have the same yintercept as uninhibited enzyme (since V_{max} is unaffected by competitive inhibitors the inverse of Vmax also doesn't change) but there are different slopes and xintercepts between the two data sets. Noncompetitive inhibition produces plots with the same xintercept as uninhibited enzyme (K_{m} is unaffected) but different slopes and yintercepts. Uncompetitive inhibition causes different intercepts on both the y and x axes but the same slope. See also
Double Reciprocal Plot References


This article is licensed under the GNU Free Documentation License. It uses material from the Wikipedia article "LineweaverBurk_plot". A list of authors is available in Wikipedia. 