Topic World Chromatography

Chromatography enables us to separate, identify and thus understand complex substances. Whether in the food industry, pharmaceutical research or environmental analysis - chromatography opens up a treasure trove of information about the composition and quality of our samples. Discover the fascinating world of chromatography!

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Protein Purification Handbooks Protein Purification Handbooks

Protein Purification Handbooks

GE Healthcare’s Life Sciences business offers principles and methodology handbooks covering a wide range of techniques and applications including sample preparation, protein purification, electrophoresis, blotting, and cell culture. Whether you’re a beginner or an expert, our handbooks are a great reference for practical tips and in-depth information about common methodologies used in the lab. Strategies for Protein Purification Handbook The development of generic purification strategies has revolutionized protein purification by offering speed and simplicity at all scales. Whether protein purification is performed at microgram or milligram quantities for research or at kilogram to ton quantities for industry, there are proven strategies that can help you apply a systematic approach to purification. Affinity Chromatography Handbook Collection Affinity Chromatography Handbooks Vols. 1 to 3 present the most effective and most frequently used strategies for sample preparation and purification of proteins using affinity chromatography in the laboratory. The blend of general guidance and specific examples will be of enormous value to both novice and expert in developing a successful affinity purification strategy for a wide range of biomolecules. Affinity Chromatography Vol. 1: Antibodies Affinity Chromatography Vol. 2: Tagged Proteins Affinity Chromatography Vol. 3: Specific Groups of Biomolecules  Updated Ion Exchange Chromatography Handbook The Ion Exchange Chromatography Handbook has been recently updated to include the expanding range of Capto™ chromatography resins for lab- and large-scale protein purification. The update brings Sepharose™ and Capto IEX resins together in the same book and the content will ensure you get the most from your purification runs. In addition to a new chapter on large-scale purification, the handbook describes each product and how to use them, and provides useful hints and tips for getting great results from your ion exchange chromatography runs. This handbook is an excellent resource on the topic of ion exchange chromatography, whether you are a first-time user or an expert. Size Exclusion Chromatography Handbook Since the introduction of Sephadex™ more than 50 yr ago, size exclusion chromatography (SEC) has played a key role in the purification of proteins and enzymes, polysaccharides, nucleic acids, and other biological macromolecules. Also known as gel filtration, SEC is the simplest and mildest of all chromatography techniques and separates molecules based on differences in size. The handbook will give you all the knowledge and details needed for successful purification runs regardless of whether you use the resin in group separation or high-resolution fractionation mode. The complete list of available handbooks can be found on gelifesciences.com/handbooks and we welcome you to download the PDF versions to start building your knowledge library. You can also checkout our knowledge videos on YouTube. https://youtu.be/FUAQKjKT99Y - The Principle of Affinity Chromatography https://youtu.be/q3fMqgT1do8 - The Principle of Ion Exchange Chromatography https://youtu.be/oV5VB5kO3tQ - Principles of Gel Filtration (Size Exclusion) Chromatography

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Topic world Gas chromatography

Gas chromatography is an essential method in analytical chemistry for the separation and analysis of volatile compounds. Due to its high resolution and sensitivity, it has become firmly established in areas such as environmental analysis, food chemistry or forensic science. GC provides precise and reliable results and enables deep insights into the chemical composition of samples.

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Topic world Gas chromatography

Gas chromatography is an essential method in analytical chemistry for the separation and analysis of volatile compounds. Due to its high resolution and sensitivity, it has become firmly established in areas such as environmental analysis, food chemistry or forensic science. GC provides precise and reliable results and enables deep insights into the chemical composition of samples.

5+ products
4 brochures
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Chromatography explained

Affinity chromatography

Affinity chromatography Affinity chromatography is a chromatographic method of separating biochemical mixtures, based on a highly specific biologic interaction such as that between antigen and antibody , enzyme and substrate , or receptor and ligand . Affinity chromatography combines the size ...

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Chiral column chromatography

Chiral column chromatography Chiral column chromatography is a variant of column chromatography , where the stationary phase is chiral instead of achiral. The enantiomer s of the same compound then differ in affinity to the stationary phase, thus they exit the column at different times. The chir ...

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Chromatographic Separation Methods in Simple Terms

Separating mixtures of substances to analyze them qualitatively and quantitatively – these are the chromatographic methods you should know about ...

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Chromatography

Chromatography Chromatography (from Greek χρώμα:chroma, colour and γραφειν:"grafein" to write) is the collective term for a family of laboratory techniques for the separation of mixtures . It involves passing a mixture dissolved in a "mobile phase" through a stationary phase, which separates ...

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Column chromatography

Column chromatography Column chromatography in chemistry is a method used to purify individual chemical compounds from mixtures of compounds. It is often used for preparative applications on scales from micrograms up to kilograms. The classical preparative chromatography column is a glass tube ...

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Fast protein liquid chromatography

Fast protein liquid chromatography Fast Protein Liquid Chromatography, usually referred to as FPLC, is a form of column chromatography used to separate or purify proteins from complex mixtures by using Ion Chromatography . It is very commonly used in biochemistry and enzymology . Columns used w ...

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Gas-liquid chromatography

Gas-liquid chromatography Gas-liquid chromatography (GLC), or simply gas chromatography (GC), is a type of chromatography in which the mobile phase is a carrier gas, usually an inert gas such as helium or an unreactive gas such as nitrogen , and the stationary phase is a microscopic layer of li ...

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Gel Permeation Chromatography

Gel Permeation Chromatography Gel permeation chromatography (GPC) is a separation technique based on hydrodynamic volume (size in solution). Molecules are separated from one another based on differences in molecular size. This technique is often used for polymer molecular weight determination. GP ...

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High performance liquid chromatography

High performance liquid chromatography High-performance liquid chromatography (HPLC) is a form of column chromatography used frequently in biochemistry and analytical chemistry . It is also sometimes referred to as high-pressure liquid chromatography. HPLC is used to separate components of a mixt ...

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Hydrophilic interaction liquid chromatography

Hydrophilic interaction liquid chromatography HILIC (HydrophILic Interaction Chromatography or Hydrophilic Interaction LIquid Chromatography) is a version of normal phase liquid chromatography a name suggested by Dr. Andrew Alpert in his 1990 paper on the subject (J. Chromatogr. 499 (1990) 177) whe ...

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Mixed-mode chromatography

Mixed-mode chromatography Mixed-mode chromatography is a type of chromatography . In liquid chromatography , several modes of separation are commonly applied to resolve a mixture of different molecules. Ion-exchange chromatography , normal phase chromatography , reversed phase chromatography , size ...

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Size exclusion chromatography

Size exclusion chromatography Size exclusion chromatography (SEC) is a chromatographic method in which particles are separated based on their size, or in more technical terms, their hydrodynamic volume . It is usually applied to large molecule s or macromolecular complexes such as proteins and ind ...

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Supercritical fluid chromatography

Supercritical fluid chromatography Supercritical Fluid Chromatography (SFC) is a robust and easy-to-use form of normal phase chromatography ideally suited to the analysis and purification of low to moderate molecular weight, thermally labile molecules. It is especially suited to the separation of ...

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The Separation Principle Behind Chromatography in Simple Terms

An overview of the physical and chemical basics and more ...

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Thin layer chromatography

Thin layer chromatography Thin layer chromatography (TLC) is a chromatography technique used to separate chemical compounds . It involves a stationary phase consisting of a thin layer of adsorbent material, usually silica gel , aluminium oxide , or cellulose immobilised onto a flat, inert carrie ...

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