01-06-2011

Abstract The RealTime ready Configurator is a web-based configuration and ordering portal for function tested, custom RT-qPCR assays based on Universal ProbeLibrary (UPL) technology. It contains consolidated gene annotation information derived from three key public gene annotation resources ...

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01-06-2011

Abstract On the RealTime ready Configurator [1], we offer function tested human, mouse, and rat qPCR assays for gene expression quantification based on the unique Universal ProbeLibrary technology (UPL) [2]. The Universal ProbeLibrary is a set of short hydrolysis probes (8–9-mers) containing ...

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16-06-2010

Bioscience Genotyping and Mapping Division, The Australian National University SNPs were easily detected with the Amplifluor® System from Millipore Detailed protocol and instrument settings are shown for this assay BMG LABTECH’s FLUOstar OPTIMA has proved to be an excellent reader for this ...

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25-01-2010

Introduction Although congenital heart malformations are the most common birth defects in humans, the underlying patho­mechanisms remain widely unknown. Through linkage analyzes and candidate-gene approaches, several gene mutations causing congenital heart defects (e.g., CITED2, GATA4, ...

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18-01-2010

Introduction Advancements in high-throughput gene expression analysis have set a new demand for automated isolation of cellular RNA from high sample numbers in parallel. The availability of the innovative MagNA Pure 96 System together with dedicated kits, protocols, and consumables sets a ...

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18-01-2010

Introduction The MagNA Pure 96 System is a brand-new system recently introduced to the market: it is a fully automated, ultra-fast system for high-throughput isolation of high- quality nucleic acids from various sample types that can handle sample volumes of up to 1 ml. The main features ...

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04-01-2010

Pyrosequencing of complementary DNA-PCR ampli­cons was used to determine comprehensive major histocompatibility complex (MHC) class-I genotypes in primates that provide essential preclinical models for studies of infectious disease, vaccine development, and transplantation. By sequence-based ...

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21-12-2009

FAQ: When was gene silencing first described? HelpCorner: In 2006, Andrew Z. Fire and Craig C. Mello received the Nobel Prize in Physiology and Medicine for their discovery of “RNA interference - gene silencing by double-stranded RNA”. “To their surprise, they found that double-stranded RNA ...

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21-12-2009

Introduction High Resolution Melting (HRM) is a novel and promising screening technique used for mutation analysis and detection. Its application prevents the need to sequence all the exons of a gene when looking for novel mutations. The method is therefore time- and cost-effective for the ...

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16-12-2009

Introduction The novel small molecule inhibitors are often substrates of ABC transporters [1] which influence the pharmacokinetic parameters of the drug candidates and cause drug resistance of tumor cells [2,3]. Therefore expression profiling of ABC transporter genes in cell lines used for ...

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