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Article 1 - 5 of 49 |
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Nature's Ingenuity Shows the Way
FusionOpticsTM - The New Dimension of Stereomicroscopy
Up to 80 percent of our experience of our visual environment takes place via our visual perception. Without spatial vision, we would hardly be able to stay oriented. In recent decades, the neurosciences have gained many insights into the complex processes by which our brain's visual cortex and cerebral cortex do the fascinating job of processing the signals originating from the eyes into an image. A study carried out jointly by Leica Microsystems and the Institute of Neuroinformatics at the University of Zurich and Swiss Federal Institute of Technology showed how flexible and powerfull our brain joins visual signals to create an optimal spatial image. The results provided the basis for an innovation in stereomicroscopy which, in terms of resolution and focus depth, has broken through limits that were previously impossible to overcome....
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Effects of Passage Number on Cell Line Transfection
An important factor in the success of any transfection experiment is the quality of the cells. As a key experimental component, the cell line can be the factor with the highest variability, affecting the reliability and reproducibility of results....
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Pathogen detection with VYOO(TM)
Sepsis is the most common cause of death in hospitalized patients worldwide. Inadequate antibiotic therapy represents an independent determinant of patients' mortality. Promptness of pathogen detection and the appliance of appropriate antibiotics are the cornerstones of management of severe bacterial infections. Furthermore, resistance to antibiotics is an increasing public health problem that can be attributed largely to their overuse. Thus, rapid diagnostic tests with both superior positive and negative predictive values over current techniques are sorely needed....
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Molecular diagnostic test applied to ascitic fluid
Spontaneous bacterial peritonitis (SBP) is a common and life-threatening complication of patients with ascites and cirrhosis of the liver. The mortality accounts for up to 30-50% and such patients die within several hours after infection. An early diagnosis and subsequent antibiotic therapy are prerequisites for the survival of the patients. The clinical standard for a diagnosis of SBP is the increased number of polymorphic neutrophilic cells in the ascites (> 250/l), independent of the results obtained with ascites cultures. An increased cell count is not detectable in all patients. Positive ascites cultures are typically found in 40-90% of all SBP cases....
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Efficacy Comparison of Transfection Reagents in a Collecting Duct Cell Line
The cortical collecting duct regulates ion and water homeostasis in the kidney. A cell line denoted M-1 has been established from the cortical collecting duct of a transgenic mouse that exhibits many functional properties of the original tissue. While this cell line is valuable for in vitro studies, the cells are not easily transfected. Frequently, adenovirus-mediated gene transfer is used. As adenovirus production requires several additional steps, we prefer chemical methods to achieve recombinant protein expression in M-1 cells. Previous attempts to transfect this cell line were unsuccessful with far less than 10% of transfectants, although different reagents were tried. Here we compare three commercially available transfection reagents in terms of the percentage of transfected cells and the suitability for using a reporter construct in M-1 cells....
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