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Automated Performance Verification

Advances in UV/VIS Spectroscopy

The sealed reference material CRM containing cuvettes of the CertiRef module

The CertiRef module mounted on top of a METTLER TOLEDO UV7 spectrophotometer

Performance verification is essential to ensure accurate and reliable instrument performance. Widely accepted guidelines for performance verification of spectrophotometers are described in the US Pharmacopeia chapter, USP 857

The recommended tests include the check of photometric accuracy and repeatability, wavelength accuracy and repeatability, instrument resolution as well as stray light measurement.

The US Pharmacopeia has introduced the USP 857 chapter on ultra-violet visible spectroscopy and adapted the test for stray light. Here, we compare the methods for measuring stray light according to the current and previous version of the US Pharmacopeia, assess the advantages of the new test and introduce automated optical performance verification.

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