Steps to razor-sharp imaging inside 3D cell models

Tissue clearing and objective selection explained in simple terms

Spheroids and organoids are excellent in vivo models for complex biochemical processes. However, light scattering of internal cellular compartments is a major challenge for imaging techniques. A variety of tissue clearing techniques are available to make tissue models transparent. Find out what you need to consider in order to obtain high-resolution images without affecting model morphology.

Download this free application note and learn how to:

  • optimally prepare 200-300 µm thick spheroids and organoids for 3D imaging in a single step, using tissue clearing techniques
  • obtain razor-sharp images thanks to an objective with a matching refractive index,
  • perform quantitative 3D analyses with smart cell analysis software such as NoviSight.

Facts, background information, dossiers
More about Olympus