Spheroids and organoids are excellent in vivo models for complex biochemical processes. However, light scattering of internal cellular compartments is a major challenge for imaging techniques. A variety of tissue clearing techniques are available to make tissue models transparent. Find out what you need to consider in order to obtain high-resolution images without affecting model morphology.
Download this free application note and learn how to:
- optimally prepare 200-300 µm thick spheroids and organoids for 3D imaging in a single step, using tissue clearing techniques
- obtain razor-sharp images thanks to an objective with a matching refractive index,
- perform quantitative 3D analyses with smart cell analysis software such as NoviSight.