Validation of Covalent Immune Recruiters (CIRs) with Synthetic Tumor Immunotherapeutic Applications

Learning Objectives:

• Discover novel trifunctional CIRs that can selectively label serum antibodies and direct immune responses to specific target proteins

• Use of label-free binding assays to obtain kinetic information to quantify covalent labeling of antibodies and evaluate selectivity

• Monitoring target protein binding and antibody recruitment under relevant equilibrium conditions

• Validation of CIRs specificity and selectively in vivo