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Superdex 75 Increase column use on ÄKTA pure
SEC Increase prepacked column formats
Comparison between Superose 6 Increase and Superdex 200 Increase. Superose 6 Increase gives higher resolution for proteins with Mr ≥ 440 000, while proteins of lower molecular weight are better resolved on Superdex 200 Increase
Comparison between Superose 6 Increase and Superdex 200 Increase. Superose 6 Increase gives higher resolution for proteins with Mr ≥ 440 000, while proteins of lower molecular weight are better resolved on Superdex 200 Increase
The new generation size exclusion chromatography columns called “Increase” from GE Healthcare offer improved performance than their predecessors.
Comprising smaller, more rigid beads with a narrower particle size distribution and a higher selectivity than their predecessors, they offer:
The columns are tolerance to repeated harsh cleaning procedures at high pH, giving long column lifetime and minimal carry-over
With the addition of Superdex™ 30 Increase in June 2017, you now have four choices of the new generation SEC resin for small-scale preparative purification and analysis (4 to 500 µl):
Three of the resins (Superdex 75 Increase, Superdex 200 Increase and Supersoe 6 Increase) are available prepacked in high-performance columns of three different sizes:10 mm x 300 mm (24 ml column volume), 5 mm x 150 mm (3 ml column volume) and 3.2 mm x 300 mm (2.4 ml column volume). Superdex 30 Increase is available as 10 mm x 300 mm and 3.2 mm x 300 mm columns. All columns are made of glass to allow easier visual inspection of the packed bed. The columns have fittings for simple connection to ÄKTA™ chromatography systems or equivalent high-performance systems.
Three different column dimensions are available:
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