New Generation SEC Columns for Small-Scale Preparative Purification and Analysis of Proteins

The new generation size exclusion chromatography columns called “Increase” from GE Healthcare offer improved performance than their predecessors. 

Comprising smaller, more rigid beads with a narrower particle size distribution and a higher selectivity than their predecessors, they offer:

• Reduced runtimes with maintained resolution for results faster
• Higher resolution for improved purity
• Higher lot to lot consistency

The columns are tolerance to repeated harsh cleaning procedures at high pH, giving long column lifetime and minimal carry-over

With the addition of Superdex™ 30 Increase in June 2017, you now have four choices of the new generation SEC resin for small-scale preparative purification and analysis (4 to 500 µl):

• For peptides and other small biomolecules: Superdex 30 Increase (Fractionation range Mr 100 to 7000)
• For recombinant tagged proteins: Superdex 75 Increase (Fractionation range Mr 3000 to 70 000)
• For MAbs and other antibodies: Superdex 200 Increase (Fractionation range Mr 10 000 to 600 000
• For larger proteins and protein complexes: Superose™ 6 Increase (Fractionation range Mr 5000 to 5 000 000)

Three of the resins (Superdex 75 Increase, Superdex 200 Increase and Supersoe 6 Increase) are available prepacked in high-performance columns of three different sizes:10 mm x 300 mm (24 ml column volume), 5 mm x 150 mm (3 ml column volume) and 3.2 mm x 300 mm (2.4 ml column volume). Superdex 30 Increase is available as 10 mm x 300 mm and 3.2 mm x 300 mm columns. All columns are made of glass to allow easier visual inspection of the packed bed. The columns have fittings for simple connection to ÄKTA™ chromatography systems or equivalent high-performance systems.

Three different column dimensions are available:

• The 10 × 300 mm column is well suited for high resolution separations for both preparative and analytical purposes, with sample volumes from 25 µl to 500 µl
• For smaller sample volumes (4–50 µl) and high resolution preparations and analyses, the 3.2 × 300 mm column is the best choice
• The shorter 5 × 150 mm column is suitable for quick purity checks and rapid screening when the highest resolution is not needed

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