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Superdex 75 Increase column use on ÄKTA pure
SEC Increase prepacked column formats
Comparison between Superose 6 Increase and Superdex 200 Increase. Superose 6 Increase gives higher resolution for proteins with Mr ≥ 440 000, while proteins of lower molecular weight are better resolved on Superdex 200 Increase
Comparison between Superose 6 Increase and Superdex 200 Increase. Superose 6 Increase gives higher resolution for proteins with Mr ≥ 440 000, while proteins of lower molecular weight are better resolved on Superdex 200 Increase
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The new generation size exclusion chromatography columns called “Increase” from GE Healthcare offer improved performance than their predecessors.
Comprising smaller, more rigid beads with a narrower particle size distribution and a higher selectivity than their predecessors, they offer:
The columns are tolerance to repeated harsh cleaning procedures at high pH, giving long column lifetime and minimal carry-over
With the addition of Superdex™ 30 Increase in June 2017, you now have four choices of the new generation SEC resin for small-scale preparative purification and analysis (4 to 500 µl):
Three of the resins (Superdex 75 Increase, Superdex 200 Increase and Supersoe 6 Increase) are available prepacked in high-performance columns of three different sizes:10 mm x 300 mm (24 ml column volume), 5 mm x 150 mm (3 ml column volume) and 3.2 mm x 300 mm (2.4 ml column volume). Superdex 30 Increase is available as 10 mm x 300 mm and 3.2 mm x 300 mm columns. All columns are made of glass to allow easier visual inspection of the packed bed. The columns have fittings for simple connection to ÄKTA™ chromatography systems or equivalent high-performance systems.
Three different column dimensions are available:
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