i3 DMC PrA 1.0mL

The purification of sensitive antibodies often fails during the acidic elution step. The i3 DMC PrA® combines Protein A affinity with Digital Membrane Chromatography (DMC) and replaces elution at low pH with a controlled electrical voltage. This allows you to obtain monoclonal antibodies, Fc-containing molecules, and other Protein A-binding molecules in their native conformation, without aggregation or fragmentation.

Digital Membrane Chromatography: Elution Exactly Where Binding Occurs

The membrane is located between two gold-coated electrode membranes and is connected to the i3 DMC Control digital control unit via integrated contacts. The control unit applies a voltage to the membrane adsorbent, thereby altering the local electrochemical conditions. These changes influence the interaction between the membrane and the target molecule, causing it to dissociate directly on the membrane.

This reduces harsh process conditions, helps preserve protein integrity, and makes samples available more quickly for downstream analyses. Neutralization and buffer exchange are no longer necessary.

Two formats for Protein A membrane chromatography

• i3 DMC PrA® 0.1 mL: Bed volume 0.1 mL, IgG1 binding capacity up to 5 mg, two-layer cellulose membrane with open pores (0.2 µm) for convective flow. Suitable for research and development.
• i3 DMC PrA® 1.0 mL (new): Bed volume 1.0 mL, dynamic binding capacity of up to 30 mg of polyclonal IgG, 90% recovery for low-abundance samples. More purified material for expanded analytical characterization and early-stage development.

Both formats follow the same separation principle and workflow. This allows the solution to grow with your project requirements.

Applications and Integration into Existing FPLC Systems

• Antibody purification from cell culture supernatants and other complex sample matrices
• Sample preparation for mass spectrometry, ELISA, BLI, and SPA
• Preparation for early-stage in vivo pharmacology studies
• Up to ten purification cycles with a suitable CIP protocol

The system connects to your FPLC system via a Luer-Lock connection. Establish the electrical connection, select a protocol, trigger elution, and collect the eluate: The workflow consists of five simple steps.

Test the technology with your samples. Request a sample kit with no obligation using the form, or consult with DMC experts.