Topic World Chromatography

Chromatography enables us to separate, identify and thus understand complex substances. Whether in the food industry, pharmaceutical research or environmental analysis - chromatography opens up a treasure trove of information about the composition and quality of our samples. Discover the fascinating world of chromatography!

Chromatography products and catalogs

Chromatography products

Holistic Multi-Column Chromatography Solution Holistic Multi-Column Chromatography Solution

Holistic Multi-Column Chromatography Solution

Flexible operation from parallel batches through SMB to integrated continuous processing
Up to 90% resin savings due to higher productivity ✓ Up to 40% solvent savings due to better utilization of the column bed ✓ Seamless scale-up from mg- to kg-range of purified product ✓
chromatography systems
High purity bottles made of PFA fluoropolymer with GL45 thread High purity bottles made of PFA fluoropolymer with GL45 thread

High purity bottles made of PFA fluoropolymer with GL45 thread

Multifunctional, inert and reusable
Can be used with GL45 screw caps and adapters ✓ For high purity applications ✓ Preserves sample integrity ✓
laboratory bottles
Reliable HPLC results with bioinert columns Reliable HPLC results with bioinert columns

Reliable HPLC results with bioinert columns

Highly accurate results from the first injection: bioinert coated YMC Accura (U)HPLC columns
Exceptional peak shapes with high sensitivities ✓ ✓ Excellent recoveries without column preconditioning ✓ ✓ Superior reproducibility and no carry-over effects ✓ ✓
UHPLC columns
YMC HT Glass Columns YMC HT Glass Columns

YMC HT Glass Columns

Pack your own MPLC glass columns, ideal for temperature sensitive applications
Temperature range of 4-70°C ✓ Easy handling and quick column packing ✓ Suitable for all resins ✓
chromatography columns
Efficient Purification of Biomolecules with High Performance Ion Exchange Resins Efficient Purification of Biomolecules with High Performance Ion Exchange Resins

High-performance ion exchange resins for biochromatography

Optimised purification of proteins and other biomolecules
Ion exchange resins specifically designed for biochromatography ✓ Increased productivity ✓ Full compliances with GMP requirements ✓ Reduced processing costs
chromatography resins
Fast & Efficient Protein Purification with Cytiva’s Self-Cleaving Resin Fast & Efficient Protein Purification with Cytiva’s Self-Cleaving Resin

Fast & Efficient Protein Purification with Cytiva’s Self-Cleaving Resin

chromatography resins
An ultrapure Type 1 water purification system designed with you in mind An ultrapure Type 1 water purification system designed with you in mind

An ultrapure Type 1 water purification system designed with you in mind

ultrapure water systems
Integrated Ultrapure & Pure Water Systems Designed to simplify your daily lab life Integrated Ultrapure & Pure Water Systems Designed to simplify your daily lab life

Integrated Ultrapure & Pure Water Systems Designed to simplify your daily lab life

water treatment systems
Expand your knowledge in liquid chromatography with tailor-made training! Expand your knowledge in liquid chromatography with tailor-made training!

Advanced training in HPLC: Flexible and customised

trainings

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Chromatography explained

Affinity chromatography

Affinity chromatography Affinity chromatography is a chromatographic method of separating biochemical mixtures, based on a highly specific biologic interaction such as that between antigen and antibody , enzyme and substrate , or receptor and ligand . Affinity chromatography combines the size ...

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Chiral column chromatography

Chiral column chromatography Chiral column chromatography is a variant of column chromatography , where the stationary phase is chiral instead of achiral. The enantiomer s of the same compound then differ in affinity to the stationary phase, thus they exit the column at different times. The chir ...

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Chromatographic Separation Methods in Simple Terms

Separating mixtures of substances to analyze them qualitatively and quantitatively – these are the chromatographic methods you should know about ...

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Chromatography

Chromatography Chromatography (from Greek χρώμα:chroma, colour and γραφειν:"grafein" to write) is the collective term for a family of laboratory techniques for the separation of mixtures . It involves passing a mixture dissolved in a "mobile phase" through a stationary phase, which separates ...

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Column chromatography

Column chromatography Column chromatography in chemistry is a method used to purify individual chemical compounds from mixtures of compounds. It is often used for preparative applications on scales from micrograms up to kilograms. The classical preparative chromatography column is a glass tube ...

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Fast protein liquid chromatography

Fast protein liquid chromatography Fast Protein Liquid Chromatography, usually referred to as FPLC, is a form of column chromatography used to separate or purify proteins from complex mixtures by using Ion Chromatography . It is very commonly used in biochemistry and enzymology . Columns used w ...

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Gas-liquid chromatography

Gas-liquid chromatography Gas-liquid chromatography (GLC), or simply gas chromatography (GC), is a type of chromatography in which the mobile phase is a carrier gas, usually an inert gas such as helium or an unreactive gas such as nitrogen , and the stationary phase is a microscopic layer of li ...

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Gel Permeation Chromatography

Gel Permeation Chromatography Gel permeation chromatography (GPC) is a separation technique based on hydrodynamic volume (size in solution). Molecules are separated from one another based on differences in molecular size. This technique is often used for polymer molecular weight determination. GP ...

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High performance liquid chromatography

High performance liquid chromatography High-performance liquid chromatography (HPLC) is a form of column chromatography used frequently in biochemistry and analytical chemistry . It is also sometimes referred to as high-pressure liquid chromatography. HPLC is used to separate components of a mixt ...

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Hydrophilic interaction liquid chromatography

Hydrophilic interaction liquid chromatography HILIC (HydrophILic Interaction Chromatography or Hydrophilic Interaction LIquid Chromatography) is a version of normal phase liquid chromatography a name suggested by Dr. Andrew Alpert in his 1990 paper on the subject (J. Chromatogr. 499 (1990) 177) whe ...

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Mixed-mode chromatography

Mixed-mode chromatography Mixed-mode chromatography is a type of chromatography . In liquid chromatography , several modes of separation are commonly applied to resolve a mixture of different molecules. Ion-exchange chromatography , normal phase chromatography , reversed phase chromatography , size ...

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Size exclusion chromatography

Size exclusion chromatography Size exclusion chromatography (SEC) is a chromatographic method in which particles are separated based on their size, or in more technical terms, their hydrodynamic volume . It is usually applied to large molecule s or macromolecular complexes such as proteins and ind ...

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Supercritical fluid chromatography

Supercritical fluid chromatography Supercritical Fluid Chromatography (SFC) is a robust and easy-to-use form of normal phase chromatography ideally suited to the analysis and purification of low to moderate molecular weight, thermally labile molecules. It is especially suited to the separation of ...

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The Separation Principle Behind Chromatography in Simple Terms

An overview of the physical and chemical basics and more ...

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Thin layer chromatography

Thin layer chromatography Thin layer chromatography (TLC) is a chromatography technique used to separate chemical compounds . It involves a stationary phase consisting of a thin layer of adsorbent material, usually silica gel , aluminium oxide , or cellulose immobilised onto a flat, inert carrie ...

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