PGreen

Additional recommended knowledge

Daily Sensitivity Test

How to quickly check pipettes?

How to ensure accurate weighing results every day?

pGreen

The pGreen plasmids were first described in 2000, pGreen: a versatile and flexible binary Ti vector for Agrobacterium-mediated plant transformation Plant Molecular Biology 42: 819-832, 2000. The supporting web page www.pGreen.ac.uk provides supplementary information and ongoing support to researchers to request their plasmid resources. As these plasmids have been taken up by the research community, that plasmids have been developed, expanding the resources available to the community.

Researchers are encouraged to contribute to this research community by submitting their vector sequence to genbank and providing a description of the plasmid on this site. Resources such as these are best shared.

pGreenI and pGreenII

pGreen AJ007829

The original pGreen plasmid

pGreenII EF590266

Plasmid backbone modification to improve plasmid stability (review manuscript in preparation)

T-DNA regions

No transformation Selection

pGreenII 0000 EU048862

minimal T-DNA with Left and Right border, Lac Z gene for blue/while selection during cloning multiple cloning site derived from pBluescript

pGreenII 62-SK EU048868

Derived from pGreenII 0000, the LacZ blue/white cloning selection has been replaced with a 35S-MCS-CaMV cassette that allows the insertion of a gene of interest into a 35S over-expression cassette. The MCS is derived from pBluescript.

pGreenII 0800-LUC EU048863

Kanamycin Selection

pGreenII 0029 EU048864

Derived from pGreenII 0000, a nos-kan cassette has been inserted into the HapI site of the Left Border, providing resistance to kanamycin dring plant transformation selection.

pGreenII 0029 62-SK EU048865

Derived from pGreenII 0029, the LacZ blue/white cloning selection has been replaced with a 35S-MCS-CaMV cassette that allows the insertion of a gene of interest into a 35S over-expression cassette. The MCS is derived from pBluescript.

Hygromycin Selection

pGreenII 0179 EU048866

Derived from pGreenII 0000, a 35S-hyg cassette has been inserted into the HapI site of the Left Border, providing resistance to hygromycin during plant transformation selection.

Bialaphos Selection

pGreenII 0229 EU048867

Derived from pGreenII 0000, a nos-bar cassette has been inserted into the HapI site of the Left Border, providing resistance to bialaphos or phosphinothricin dring plant transformation selection.

pGreenII 0229 62-SK EU048869

Derived from pGreenII 0229, the LacZ blue/white cloning selection has been replaced with a 35S-MCS-CaMV cassette that allows the insertion of a gene of interest into a 35S over-expression cassette. The MCS is derived from pBluescript.

pSoup

This is the helper plasmid that provides the replicase function for the pSa replication origin of pGreen. pSoup is tetracyclin resistant and a complementary incompatibility group such that it can co-exist with pGreen in the Agrobacterium cell.

pSoup EU048870

The original help plasmid for pSoup. pGreen will not replicte in E. coli if it is not present. The T-DNA region of pGreen (or any other T-DNA region from other vecotrs) will not be transferred to plants via Agrobacterium if this plasmid is not present.