My watch list  

Dialysis (biochemistry)

In biochemistry, dialysis is the process of separating molecules in solution by the difference in their rates of diffusion through a semipermeable membrane, such as dialysis tubing.

Dialysis is a common laboratory technique, and operates on the same principle as medical dialysis. Typically a solution of several types of molecules is placed into a semipermeable dialysis bag, such as a cellulose membrane with pores, and the bag is sealed. The sealed dialysis bag is placed in a container of a different solution, or pure water. Molecules small enough to pass through the tubing (often water, salts and other small molecules) tend to move into or out of the dialysis bag, in the direction of decreasing concentration. Larger molecules (often proteins, DNA, or polysaccharides) that have dimensions significantly greater than the pore diameter are retained inside the dialysis bag. One common reason for using this technique would be to remove the salt from a protein solution. The technique will not distinguish between proteins effectively.

See also

This article is licensed under the GNU Free Documentation License. It uses material from the Wikipedia article "Dialysis_(biochemistry)". A list of authors is available in Wikipedia.
Your browser is not current. Microsoft Internet Explorer 6.0 does not support some functions on Chemie.DE