To use all functions of this page, please activate cookies in your browser.
With an accout for my.bionity.com you can always see everything at a glance – and you can configure your own website and individual newsletter.
- My watch list
- My saved searches
- My saved topics
- My newsletter
Additional recommended knowledge
Acid-fast organisms are difficult to characterize using standard microbiological techniques (e.g. Gram staining), though they can be stained using concentrated dyes, particularly when the staining process is combined with heat. Once stained, these organisms resist the dilute acid and/or ethanol-based de-colorization procedures common in many staining protocols—hence the name acid-fast.
The high mycolic acid content of certain bacterial cell walls, like those of Mycobacterium, is responsible for the staining pattern of poor absorption followed by high retention. The most common staining technique used to identify acid-fast bacteria is the Ziehl-Neelsen stain, in which the bacteria are stained bright red and stand out clearly against a blue background. Acid-fast bacteria can also be visualized by fluorescence microscopy using specific fluorescent dyes (auramine-rhodamine stain, for example). Some bacteria may also be partially acid-fast.
Notable Acid fast structures
Very few structures are acid fast, this makes staining for acid-fastness particularly useful in diagnosis.
Online protocol examples
|This article is licensed under the GNU Free Documentation License. It uses material from the Wikipedia article "Acid-fast". A list of authors is available in Wikipedia.|