Primers

Products Primers

NGSeq 16S V3/V4 – Ultra-Clean Amplicon PCR For 16S Library Preparation - For Illumina® MiSeq System

Molzym’s NGSeq 16S V3/V4 bacterial DNA-free master mix matches the formulation by Illumina® for Amplicon PCR for 16S metagenomic sequencing with the MiSeq system more

New: Ultra-Sensitive PCR Reagents for Molecular Microbiology

Molzym offers a range of ultra-clean PCR products for 16S PCR. All kits are free of contaminating DNA enabling ultra-sensitive and reliable PCR-results more

News Primers

• Innogenetics achieves CE mark for new human papillomavirus (HPV) genotyping test

  Innogenetics announced that it has received CE marking for its new and expanded HPV genotyping test, the INNO-LiPA HPV Genotyping Extra. The new generation kit allows the genotyping of 28 different HPV genotypes, of which 18 are high-risk types for the possible development of cervical cancer. The te more

• QIAGEN and BioHelix Enter Into Licensing Agreement

  QIAGEN and privately held BioHelix have announced that they have entered into a non-exclusive worldwide license and supply agreement for BioHelix's proprietary Helicase Dependent Amplification technology (HDA). HDA is a versatile assay technology to detect and analyze biological target molecules suc more

• QIAGEN Acquires Genaco Biomedical Products, Inc.

  QIAGEN N.V. announced that it acquired all outstanding shares of Genaco Biomedical Products, Inc. (Genaco). Genaco is an early-stage company applying a proprietary PCR-based multiplexing technology, Tem-PCR, to develop Templex(TM) molecular diagnostic tests. Genaco is based in Huntsville, Alabama. more

White papers Primers

• Fast and Sensitive Detection of Viral Targets in Quantitative RT-PCR Using the Universal One-Step qRT-PCR Master (ROX)
  

  For qRT-PCR assays it is a common procedure to perform two individual work steps. First, RNA is transcribed into cDNA, and then the target to be quantified is amplified separately. This two-step approach is believed to result in highest sensitivity o more

• Major Histocompatibility Complex Genotyping with Massively Parallel Pyrosequencing
  

  Macaques provide essential preclinical models for infectious disease, vaccine, biodefense, and transplantation research. Unfortunately, they also have the most complex MHC genetics of any primate species described to date, and existing methods for MH more

• Rare Variants of IFIH1 Protect Against Type-1 Diabetes
  

  Nejentsev and co-workers have found that rare alleles of all associated IFIH1 polymorphisms consistently protect from type-1 diabetes, whereas IFIH1 alleles carried by the majority of the population predispose to the disease. This observation suggest more

Publications Primers

• Instances of erroneous DNA barcoding of metazoan invertebrates: Are universal cox1 gene primers too “universal”?

  by Monika Mioduchowska, Michał Jan Czyż, Bartłomiej Gołdyn, Jarosław Kur, Jerzy Sell The cytochrome c oxidase subunit I (cox1) gene is the main mitochondrial molecular marker playing a pivotal role in phylogenetic research and is a crucial barcode sequence. Folmer’s “universal” primers designed t more

• Viruses, Vol. 10, Pages 260: Blackcurrant Leaf Chlorosis Associated Virus: Evidence of the Presence of Circular RNA during Infections

  Viruses, Vol. 10, Pages 260: Blackcurrant Leaf Chlorosis Associated Virus: Evidence of the Presence of Circular RNA during Infections Viruses doi: 10.3390/v10050260 Authors: Delano James James Phelan Daniel Sanderson Blackcurrant leaf chlorosis associated virus (BCLCaV) was detect more

• Touchdown Polymerase Chain Reaction (PCR)

  "Touchdown polymerase chain reaction (PCR)" is a method to decrease off-target priming and hence to increase the specificity of PCRs. In touchdown PCR the temperature selected for the annealing step is initially set 5°C–10°C higher than the calculated Tm of the primers. Annealing under conditions o more