Primers

Companies Primers

Products Primers

NGSeq 16S V3/V4 – Ultra-Clean Amplicon PCR For 16S Library Preparation - For Illumina® MiSeq System

Molzym’s NGSeq 16S V3/V4 bacterial DNA-free master mix matches the formulation by Illumina® for Amplicon PCR for 16S metagenomic sequencing with the MiSeq system more

New: Ultra-Sensitive PCR Reagents for Molecular Microbiology

Molzym offers a range of ultra-clean PCR products for 16S PCR. All kits are free of contaminating DNA enabling ultra-sensitive and reliable PCR-results more

News Primers

• Innogenetics achieves CE mark for new human papillomavirus (HPV) genotyping test

  Innogenetics announced that it has received CE marking for its new and expanded HPV genotyping test, the INNO-LiPA HPV Genotyping Extra. The new generation kit allows the genotyping of 28 different HPV genotypes, of which 18 are high-risk types for the possible development of cervical cancer. The te more

• QIAGEN and BioHelix Enter Into Licensing Agreement

  QIAGEN and privately held BioHelix have announced that they have entered into a non-exclusive worldwide license and supply agreement for BioHelix's proprietary Helicase Dependent Amplification technology (HDA). HDA is a versatile assay technology to detect and analyze biological target molecules suc more

• QIAGEN Acquires Genaco Biomedical Products, Inc.

  QIAGEN N.V. announced that it acquired all outstanding shares of Genaco Biomedical Products, Inc. (Genaco). Genaco is an early-stage company applying a proprietary PCR-based multiplexing technology, Tem-PCR, to develop Templex(TM) molecular diagnostic tests. Genaco is based in Huntsville, Alabama. more

White papers Primers

• Rare Variants of IFIH1 Protect Against Type-1 Diabetes
  

  Nejentsev and co-workers have found that rare alleles of all associated IFIH1 polymorphisms consistently protect from type-1 diabetes, whereas IFIH1 alleles carried by the majority of the population predispose to the disease. This observation suggest more

• Mutation Scanning Using High Resolution Melting or dHPLC: a Performance Comparison Study
  

  High Resolution Melting (HRM) is a novel and promising screening technique used for mutation analysis and detection. Its application prevents the need to sequence all the exons of a gene when looking for novel mutations. The method is therefore time- more

• High-Throughput Cardiac Gene Expression Analysis Using the Universal ProbeLibrary and the Novel LightCycler® 1536 Real-Time PCR System
  

  Although congenital heart malformations are the most common birth defects in humans, the underlying patho­mechanisms remain widely unknown. Through linkage analyzes and candidate-gene approaches, several gene mutations causing congenital heart defect more

Publications Primers

• Instances of erroneous DNA barcoding of metazoan invertebrates: Are universal cox1 gene primers too “universal”?

  by Monika Mioduchowska, Michał Jan Czyż, Bartłomiej Gołdyn, Jarosław Kur, Jerzy Sell The cytochrome c oxidase subunit I (cox1) gene is the main mitochondrial molecular marker playing a pivotal role in phylogenetic research and is a crucial barcode sequence. Folmer’s “universal” primers designed t more

• Viruses, Vol. 10, Pages 260: Blackcurrant Leaf Chlorosis Associated Virus: Evidence of the Presence of Circular RNA during Infections

  Viruses, Vol. 10, Pages 260: Blackcurrant Leaf Chlorosis Associated Virus: Evidence of the Presence of Circular RNA during Infections Viruses doi: 10.3390/v10050260 Authors: Delano James James Phelan Daniel Sanderson Blackcurrant leaf chlorosis associated virus (BCLCaV) was detect more

• Touchdown Polymerase Chain Reaction (PCR)

  "Touchdown polymerase chain reaction (PCR)" is a method to decrease off-target priming and hence to increase the specificity of PCRs. In touchdown PCR the temperature selected for the annealing step is initially set 5°C–10°C higher than the calculated Tm of the primers. Annealing under conditions o more