Topic World Chromatography

Chromatography enables us to separate, identify and thus understand complex substances. Whether in the food industry, pharmaceutical research or environmental analysis - chromatography opens up a treasure trove of information about the composition and quality of our samples. Discover the fascinating world of chromatography!

Chromatography brochures

Chromatography news

Chromatography expert knowledge

Chromatography white papers

Chromatography webinars

Chromatography videos

Flexible and Modular: The Complete FPLC Solution for All Types of Protein Purification Flexible and Modular: The Complete FPLC Solution for All Types of Protein Purification

Flexible and Modular: The Complete FPLC Solution for All Types of Protein Purification

With its flexible AZURA FPLC (fast protein liquid chromatography) systems, KNAUER offers complete solutions for the purification of large biomolecules such as proteins, nucleotides and peptides. From size exclusion chromatography (SEC) to affinity chromatography (AC), ion exchange chromatography (IEX) and hydrophobic interaction chromatography (HIC), you can get it all from a single source: KNAUER. All you need for FPLC from a single source Configure your AZURA protein purification system according to your own requirements. Features such as automatic sample injection via autosampler, valves for column, buffer and sample selection as well as fraction collection allow you to automate the purification process. The new Sepapure FPLC columns from KNAUER are available as ready-to-use cartridges and as bulk material. This gives you high quality at a surprisingly low price. Maximum flexibility is also ensured by the AZURA FPLC systems’ compatibility with the columns of other manufacturers. It is possible to freely configure all system modules and add extra ones to create the best system for every purification task. The choices include: Pumps for the buffer and the sample Injection valves for different sample loops Column selection valves Autosamplers FPLC columns Detectors Fraction collectors The PurityChrom software More space in the lab KNAUER’s FPLC systems are strong performers and, thanks to their modular design, can be flexibly adapted to your available laboratory space. The right detector for any molecule There is a choice of different detectors to make your target molecules visible, including variable single wavelength UV, multiple wavelength UV, full spectrum diode array (DAD/3D Spectrum), conductivity and pH monitoring, fluorescence as well as refractive index. FPLC methods created in an instant The PurityChrom software allows you to create methods flexibly and have complete control of the run at all times. Methods can be easily adapted and scaled in the compact versions by changing the column volume. At the heart of the FPLC software interface lies the interactive visualization of the system, which makes controlling the components manually - even in complex systems - simple and straightforward. 

See the theme worlds for related content

Topic world Gas chromatography

Gas chromatography is an essential method in analytical chemistry for the separation and analysis of volatile compounds. Due to its high resolution and sensitivity, it has become firmly established in areas such as environmental analysis, food chemistry or forensic science. GC provides precise and reliable results and enables deep insights into the chemical composition of samples.

5+ products
4 brochures
View topic world

Topic world Gas chromatography

Gas chromatography is an essential method in analytical chemistry for the separation and analysis of volatile compounds. Due to its high resolution and sensitivity, it has become firmly established in areas such as environmental analysis, food chemistry or forensic science. GC provides precise and reliable results and enables deep insights into the chemical composition of samples.

5+ products
4 brochures
Newsletter

Get the life science industry in your inbox

From now on, don't miss a thing: Our newsletter for biotechnology, pharma and life sciences brings you up to date every Tuesday and Thursday. The latest industry news, product highlights and innovations - compact and easy to understand in your inbox. Researched by us so you don't have to.

Chromatography explained

Affinity chromatography

Affinity chromatography Affinity chromatography is a chromatographic method of separating biochemical mixtures, based on a highly specific biologic interaction such as that between antigen and antibody , enzyme and substrate , or receptor and ligand . Affinity chromatography combines the size ...

View article

Chiral column chromatography

Chiral column chromatography Chiral column chromatography is a variant of column chromatography , where the stationary phase is chiral instead of achiral. The enantiomer s of the same compound then differ in affinity to the stationary phase, thus they exit the column at different times. The chir ...

View article

Chromatographic Separation Methods in Simple Terms

Separating mixtures of substances to analyze them qualitatively and quantitatively – these are the chromatographic methods you should know about ...

View article

Chromatography

Chromatography Chromatography (from Greek χρώμα:chroma, colour and γραφειν:"grafein" to write) is the collective term for a family of laboratory techniques for the separation of mixtures . It involves passing a mixture dissolved in a "mobile phase" through a stationary phase, which separates ...

View article

Column chromatography

Column chromatography Column chromatography in chemistry is a method used to purify individual chemical compounds from mixtures of compounds. It is often used for preparative applications on scales from micrograms up to kilograms. The classical preparative chromatography column is a glass tube ...

View article

Fast protein liquid chromatography

Fast protein liquid chromatography Fast Protein Liquid Chromatography, usually referred to as FPLC, is a form of column chromatography used to separate or purify proteins from complex mixtures by using Ion Chromatography . It is very commonly used in biochemistry and enzymology . Columns used w ...

View article

Gas-liquid chromatography

Gas-liquid chromatography Gas-liquid chromatography (GLC), or simply gas chromatography (GC), is a type of chromatography in which the mobile phase is a carrier gas, usually an inert gas such as helium or an unreactive gas such as nitrogen , and the stationary phase is a microscopic layer of li ...

View article

Gel Permeation Chromatography

Gel Permeation Chromatography Gel permeation chromatography (GPC) is a separation technique based on hydrodynamic volume (size in solution). Molecules are separated from one another based on differences in molecular size. This technique is often used for polymer molecular weight determination. GP ...

View article

High performance liquid chromatography

High performance liquid chromatography High-performance liquid chromatography (HPLC) is a form of column chromatography used frequently in biochemistry and analytical chemistry . It is also sometimes referred to as high-pressure liquid chromatography. HPLC is used to separate components of a mixt ...

View article

Hydrophilic interaction liquid chromatography

Hydrophilic interaction liquid chromatography HILIC (HydrophILic Interaction Chromatography or Hydrophilic Interaction LIquid Chromatography) is a version of normal phase liquid chromatography a name suggested by Dr. Andrew Alpert in his 1990 paper on the subject (J. Chromatogr. 499 (1990) 177) whe ...

View article

Mixed-mode chromatography

Mixed-mode chromatography Mixed-mode chromatography is a type of chromatography . In liquid chromatography , several modes of separation are commonly applied to resolve a mixture of different molecules. Ion-exchange chromatography , normal phase chromatography , reversed phase chromatography , size ...

View article

Size exclusion chromatography

Size exclusion chromatography Size exclusion chromatography (SEC) is a chromatographic method in which particles are separated based on their size, or in more technical terms, their hydrodynamic volume . It is usually applied to large molecule s or macromolecular complexes such as proteins and ind ...

View article

Supercritical fluid chromatography

Supercritical fluid chromatography Supercritical Fluid Chromatography (SFC) is a robust and easy-to-use form of normal phase chromatography ideally suited to the analysis and purification of low to moderate molecular weight, thermally labile molecules. It is especially suited to the separation of ...

View article

The Separation Principle Behind Chromatography in Simple Terms

An overview of the physical and chemical basics and more ...

View article

Thin layer chromatography

Thin layer chromatography Thin layer chromatography (TLC) is a chromatography technique used to separate chemical compounds . It involves a stationary phase consisting of a thin layer of adsorbent material, usually silica gel , aluminium oxide , or cellulose immobilised onto a flat, inert carrie ...

View article