Single Base Extension

Single Base Extension (SBE) is a method for determining the identity of a nucleotide base at a specific position along a nucleic acid. The method is used to identify a single nucleotide polymorphism (SNP).

Product highlight

Efficient inline analysis for liquids and solids

Revolutionize your production: real-time Raman analysis for maximum efficiency

Freeze Drying with the Refrigerant of the Future

In the method, invented by Phillip Goelet, Michael Knapp and Stephen Anderson while at Molecular Tool, an oligonucleotide primer hybridizes to a complementary region along the nucleic acid, to form a duplex, with the primer’s terminal 3’ end directly adjacent to the nucleotide base to be identified. The oligonucleotide primer is enzymatically extended a single base by a nucleotide terminator complementary to the nucleotide being identified. The terminator prevents additional nucleotides from being incorporated.

Many different approaches can be taken for determining the identity of a terminator, including fluorescence labeling, mass labeling for mass spectrometry, measuring enzyme activity using a protein moiety, and isotope labeling.

See also

• Single Nucleotide Polymorphism

References

Philip Goelet, Michael R. Knapp, Stephen Anderson, (1999), U.S. Patent No 5,888,819. Washington, DC: U.S. Patent and Trademark Office.