SOC medium

SOC medium is a nutritionally rich bacterial growth medium, perhaps the second-most widely used medium for culture of Escherichia coli in molecular biology, the most widely used being lysogeny broth, or LB.^[1]

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Daily Sensitivity Test

Formula

• 20 g Bacto-tryptone
• 5 g Bacto-yeast extract
• 0.5 g NaCl
• 2.5 mL 1M KCl
• 1000 mL ddH2O to

Total volume = 1000mL^[2]

Ph adjustment

For maximum effectiveness, the SOC medium should be adjusted to a pH of 7.0:

• Use 10N NaOH to neutralize pH of medium at 7.0
• Autoclave medium to sterilize
• Add 20 mL of sterile 1M glucose^[3]

See also

• Lysogeny broth

References