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Ham's tissue culture medium



Ham's tissue culture medium is a growth medium for mammalian cells containing in amounts dissolved in 1 liter of triple distilled water:

L-Arginine  211 mg  
Biotin  0.024 mg
L-Histidine  21 mg  
Calcium pantothenate  0.7 mg
L-Lysine  29.3 mg  
Choline chloride  0.69 mg
L-Methionine  4.48 mg  
i-inositol  0.54 mg
L-Phenylalanine  4.96 mg  
Niacinamide  0.6 mg
L-Tryptophan  0.6 mg  
Pyridoxine hydrochloride  0.2 mg
L-Tyrosine  1.81 mg  
Riboflavin  0.37 mg
L-Alanine  8.91 mg  
Thymidine  0.7 mg
Glycine  7.51 mg   
Cyanocobalamin  1.3 mg
L-Serine  10.5 mg  
Sodium pyruvate  110 mg
L-Threonine  3.57 mg  
Lipoic acid  0.2 mg
L-Aspartic acid  13.3 mg  
CaCl2  44 mg
L-Glutamic acid  14.7 mg  
MgSO4.7H2O  153 mg
L-Asparagine  15 mg  
Glucose  1.1 g
L-Glutamine  146.2 mg  
NaCl  7.4 g
L-Isoleucine  2.6 mg  
KCl  285 mg
L-Leucine  13.1 mg  
Na2HPO4  290 mg
L-Proline  11.5 mg  
KH2PO4  83 mg
L-Valine  3.5 mg  
Phenol red  1.2 mg
L-Cysteine  31.5 mg  
FeSO4  0.83 mg
Thiamine hydrochloride  1 mg  
CuSO4.5H2O  0.0025 mg
Hypoxanthine  4 mg  
ZnSO4.7H2O  0.028 mg
Folic acid  1.3 mg  
NaHCO3  1.2 g

See also

  • Biotechnology
  • Brewing
  • Cell biology
  • Eagle's minimal essential medium (a minimum ingredient cell culture medium)
  • Fermentation (food)
  • GMEM (Glasgow's Minimal Essential Medium)
  • Murashige and Skoog medium (Plant cell culture medium)
  • RPMI (Roswell Park Memorial Institute medium), for lymph cells

Sources

  • The Nutrient Requirements of Cells
 
This article is licensed under the GNU Free Documentation License. It uses material from the Wikipedia article "Ham's_tissue_culture_medium". A list of authors is available in Wikipedia.
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