GSD microscopy

Introduction

Ground State Depletion Microscopy, or GSD Microscopy, is a technology similar to Stimulated Emission Depletion Microscopy. It can sharpen the fluorescent spot significantly and overcome the diffraction barrier. It achieves this goal by quenching the excited states of the dye molecules at the outer part of the fluorescence spot to the triplet state or other long-lived states. Therefore the fluorescence of the outer part of the spot is inhibited, and the spatial resolution is improved.