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In molecular biology and genetics, a blot is a method of transferring proteins, DNA or RNA, onto a carrier (for example, a nitrocellulose PVDF or nylon membrane). In many instances, this is done after a gel electrophoresis, transferring the molecules from the gel onto the blotting membrane, and other times adding the samples directly onto the membrane. After the blotting, the transferred proteins, DNA or RNA are then visualized by one or more different methods:
Additional recommended knowledge
Common blot methods are :
|This article is licensed under the GNU Free Documentation License. It uses material from the Wikipedia article "Blot_(biology)". A list of authors is available in Wikipedia.|