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Aseptic technique refers to a procedure that is performed under sterile conditions. This includes medical techniques and laboratory techniques, such as with microbiological cultures.
Additional recommended knowledge
Aseptic technique is the effort taken to keep patients as free from hospital micro-organisms as possible (Crow 1989). The founder of the technique is considered to be Joseph Lister. It is a method used to prevent contamination of wounds and other susceptible sites by organisms that could cause infection. This can be achieved by ensuring that only sterile equipment and fluids are used during invasive medical and nursing procedures. Ayliffe et al. (2000) suggest that there are two types of asepsis: medical and surgical asepsis. Medical or clean asepsis reduces the number of organisms and prevents their spread; surgical or sterile asepsis includes procedures to eliminate micro-organisms from an area and is practised by nurses in operating theatres and treatment areas.
Aseptic technique is the name given to the procedures used by [microbiologist]]s to prevent microbial contamination of themselves, which may result in infection, contamination of the environment they are working in (e.g. fomites), and contamination of the specimen they are working on, which is especially important when a pure culture is desired. It is used whenever specimens are to be transferred between media, for example, when subculturing. Such a procedure, using a flame sterilization method, might occur as follows:
Students of microbiology are taught the principles of aseptic technique by means of hands-on laboratory experience. Practice is essential in learning how to handle the laboratory tools without contaminating them.
|This article is licensed under the GNU Free Documentation License. It uses material from the Wikipedia article "Aseptic_technique". A list of authors is available in Wikipedia.|