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5-Bromo-4-chloro-3-indolyl phosphate



BCIP
IUPAC name 5-Bromo-4-chloro-3-indolyl phosphate
Other names BCIP
Properties
Molecular formula C8H6BrClNO4P
Molar mass 433.6 g/mol
Appearance Colorless
Solubility in water very low solubility in water or lipid
Except where noted otherwise, data are given for
materials in their standard state
(at 25 °C, 100 kPa)
Infobox disclaimer and references

5-Bromo-4-chloro-3-indolyl phosphate (BCIP) is a chemical compound used in immunology for sensitive detection of alkaline phosphatase with nitro blue tetrazolium chloride (NBT). NBT serves as the oxidant (and gives also dark blue dye) and BCIP is the AP-substrate.

BCIP (colorless)oxidation→ blue precipitate

References
     
    This article is licensed under the GNU Free Documentation License. It uses material from the Wikipedia article "5-Bromo-4-chloro-3-indolyl_phosphate". A list of authors is available in Wikipedia.
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