My watch list
With an accout for my.bionity.com you can always see everything at a glance – and you can configure your own website and individual newsletter.
- My watch list
- My saved searches
- My saved topics
- My newsletter
The Miles and Misra Method or surface variable count is a technique for counting the number of colony forming units in a bacterial suspension. It was developed by Miles and Misra in 1938.
The procedure is as follows:
- Prepare serial dilutions of the bacterial suspension as required.
- Materials required:
- A calibrated dropping pipette, delivering drops of about 0.02ml;
- 6 plates of clear nutrient agar.
- The plates are divided into numbered sectors.
- The inoculum / suspension is deposited as drops of 0.02ml from a height of 2.5cm on to the medium where it spreads over an area of 1.5 – 2.0cm diameter.
- Each of the 6 plates receives one drop of each dilution in separate numbered sectors.
- The plates are incubated for 18 – 24 hours and observed for growth.
- Sectors where more than 20 colonies are present without any confluence are utilized to make the viable counts.
- Viable count per 0.02ml for a dilution is obtained by taking the average of counts for that dilution in all the six plates.
Miles, A.A & Misra, S.S. (1938). J. Hyg. (London), 38, 732.
This article is licensed under the GNU Free Documentation License. It uses material from the Wikipedia article "Miles_and_Misra_method". A list of authors is available in Wikipedia.
Your browser is not current. Microsoft Internet Explorer 6.0 does not support some functions on Chemie.DE