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Isocaudomers are pairs of restriction enzymes that have slightly different recognition sequences but upon cleavage generate identical termini. For example the enzymes Mbo I and BamH I are isocaudomers:
Mbo I N*GATCN NCTAG*N BamH I G*GATCC CCTAG*G
N represents any of the four nucleotides. Independently of which nucleotide is present when cleaving with MboI, after cleavage with either enzyme, all termini have the central tetranucleotide - GATC. This allows fragments generated with one enzyme to anneal with fragments generated with the other enzyme.
|This article is licensed under the GNU Free Documentation License. It uses material from the Wikipedia article "Isocaudomer". A list of authors is available in Wikipedia.|