 |
|
|
|
 |
|
|
Article 1 - 5 of 108 |
|
Interference and HAMA problems
Recognizing and avoiding of false results
Immunoassays are bioanalytical methods based on antibodies. These include ELISA, EIA, RIA, Western blotting, protein arrays, lateral flow assays (immuno-chromatography), immunohistochemistry or immuno-PCR. Antibodies detect the substances (analytes) in immunoassays. This detection is always highly specific and only the analytes are detected and bound. This is the theory! Practically any antibody not only binds its analyte. It also binds several other substances, but with very different affinities....
|
|
HelpCorner - Cell Proliferation and Viability
Cell viability can be defined as the number of healthy cells in a sample. Cell viability assays are often useful when non-dividing cells (such as primary cells) are isolated and maintained in culture to determine optimal culture conditions for these populations. The most useful and straightforward method for determining viable cell number is to stain the cells with a dye such as trypan blue and count them in a hemocytometer (e.g., the Neubauer hemocytometer)....
|
|
High Pure Viral Nucleic Acid Large Volume Kit - Viral Detection in Large Sample Volumes
The isolation of nucleic acids from biological samples such as serum, plasma, or whole blood is commonly used for the detection of viral loads. It is therefore desirable to collect this low amount of nucleic acid from large sample volumes in order to measure low viral loads with routinely used qPCR and qRT-PCR principles. The High Pure Viral Nucleic Acid Large Volume Kit enables researchers to isolate viral nucleic acids from up to 2.5 ml of plasma, serum, or whole blood in 25 minutes. The isolation of viral nucleic acids is based on the well-established spin column approach....
|
|
Transcriptor High Fidelity cDNA Synthesis Kit - Accuracy Meets RT-PCR
Retroviral reverse transcriptases commonly used for cDNA synthesis exhibit a higher error rate than other DNA polymerases used in nucleic acid analysis techniques. This lack of accuracy leads to a significant number of base exchanges or frameshifts which are further propagated in a subsequent PCR reaction. With the introduction of a new high-accuracy reverse transcriptase, Roche Applied Science now offers a new tool to increase the fidelity of cDNA synthesis. The Transcriptor High Fidelity cDNA Synthesis Kit not only reduces the amount of errors in RT-PCRs; it also allows the reverse transcription of full-length cDNAs with high yield....
|
|
High-Throughput and Complex Gene Expression Validation Using the Universal ProbeLibrary and the LightCyclerŽ 480 System
During the past decade, a considerable number of transcriptome profiling analyses have been reported. High-throughput approaches such as tagging-based serial analysis of gene expression (SAGE) and hybridization-based microarray technologies have been widely performed to profile gene expression [1]. However, these techniques are either laborious or costly, or may involve advanced technologies which limit the sampling depth, thus diminishing the sensitivity of analysis. Validation of gene expression profiles can be rapidly performed using an independent technique such as quantitative polymerase chain reaction (qPCR) to eliminate false results. In most laboratories, qPCR analysis has so far been performed on a small scale....
|
|
|
|
|
|
|
|
Top