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Semen analysis



A semen analysis evaluates certain characteristics of a man's semen and the sperm contained in the semen. It may be done while investigating a couple's infertility or after a vasectomy to verify that the procedure was successful.

Additional recommended knowledge

Contents

Relation to fertility

The characteristics measured by semen analysis are only some of the factors in semen quality. One source states that 30% of men with a normal semen analysis actually have abnormal sperm function.[1] Conversely, men with poor semen analysis results may go on to father children.[2][3]

Collection methods

The most common way to collect a semen sample is through masturbation, directing the sample into a clean cup.[2]

A sample may also be collected during intercourse in a special type of condom. Collection condoms are made from silicone or polyurethane, as latex is somewhat harmful to sperm.[4]

The Catholic Church teaches that masturbation is immoral. For observant Catholics, collection condoms are the only morally permissible way to obtain semen samples. Catholics may put two or three pinholes in the collection condom to avoid violating the Catholic prohibition on contraception.[5]

A third option for collecting a sample is through coitus interruptus (withdrawal). With this technique, the man removes his penis from the woman near the end of intercourse and ejaculates into a cup. This technique is mostly only recommended for men testing for success of a vasectomy, in contrast to an investigation of infertility,[2] since the caregiver might assume that the meaning of the operation is for better quality of coitus for the man.

Finally, if a blockage in the vas deferens is suspected to impede fertility, semen can be taken directly from the epididymis. Such a sollection is called per cutaneous epididymal sperm aspiration (PESA). Alternatively, the testicular tissue itself, instead of the sperm produced can be investigated. Then, the collecting method is called TESE. [6]

Tested characteristics

Examples of parameters measured in a semen analysis are: sperm count, motility, morphology, volume, fructose level and pH.

Sperm count

Sperm count measures how many sperm are in a man's ejaculate. Anything over 20 million sperm per milliliter is considered normal.[2][1] Anything less is considered oligospermia. A vasectomy is considered successful if the sample is azoospermic, or if only rare non-motile sperm are observed (fewer than 100,000 per milliliter).[7]

Motility

The motility of the sperm is evaluated. WebMD defines normal motility as 60% of observed sperm, or at least 8 million per milliliter, showing good forward movement.[2] For instance, a man can have a total amount (sperm count) of far over the limit of 20 million sperm cells per milliliter, but still have bad quality, because too few of them are motile. However, if the sperm count is very high, then a motility of less than 60% might not matter, because the fraction might still be more than 8 million per milliliter. The other way around, a man can have a sperm count far less than 20 million sperm cells per milliliter and still have good motility, if more than 60% of those observed sperm cells show good forward movement.

The World Health Organization (WHO) criteria are that normal motility is when at least 50% of observed sperm move forward normally.[1]

Morphology

The morphology of the sperm is also evaluated. With WHO criteria, a sample is normal if 30% or more of the observed sperm have normal morphology.[1] If morphology is evaluated using the strict criteria developed by Dr. Thinus Kruger,[8] a sample is normal if 14% or more of the observed sperm have normal morphology.[1]

Volume

The volume of the sample is measured. WebMD advises that volumes between 1.0 mL and 6.5 mL are normal;[2] WHO criteria specify that any volume greater than 2.0 mL is normal. Low volume may indicate partial or complete blockage of the seminal vesicles, or that the man was born without seminal vesicles.[1]

Fructose level

The level of fructose in the semen is measured. WebMD lists normal as at least 300 mg/mL.[2] WHO specifies a normal level of 13 μmol per sample. Absence of fructose may indicate a problem with the seminal vesicles.[1]

pH

The pH of the sample is measured. WebMD lists a normal range of 7.1-8.0;[2] WHO criteria specify normal as 7.2-7.8.[1] Acidic ejaculate (lower pH value) may indicate one or both of the seminal vesicles are blocked. A basic ejaculate (higher pH value) may indicate an infection.[1] A pH value outside of the normal range is harmful to sperm.[2]

Others

The sample is tested for white blood cells. A high level of white blood cells (over 1 million per milliliter) may indicate an infection.[1]

The liquefaction time of the sample is measured. It normally takes less than 60 minutes for the sample to change from a thick gel into a liquid. An abnormally long liquefaction time may indicate an infection.[2]

Factors that influence results

Compared to samples obtained from masturbation, semen samples from collection condoms have higher total sperm counts, sperm motility, and percentage of sperm with normal morphology. For this reason, they are believed to give more accurate results when used for semen analysis.[4]

How long the man has abstained prior to providing the sample for analysis affects the results. Longer periods of abstinence correlate with poorer results - one study found that men with repeated normal results produced abnormal samples if they abstained for more than 10 days. It is recommended not to abstain for more than one or two days before providing the semen sample for analysis.[9]

If the results from a man's first sample are subfertile, they must be verified with at least two more analysis. At least 2 to 4 weeks must be allowed between each analysis.[10] Results for a single man may have a large amount of natural variation over time, meaning a single sample may not be representative of a man's average semen characteristics.[11] In addition, sperm physiologist Joanna Ellington believes that the stress of producing an ejaculate sample for examination, often in an unfamiliar setting and without any lubrication (most lubricants are somewhat harmful to sperm), may explain why men's first samples often show poor results while later samples show normal results.[3]

A man may prefer to produce his sample at home rather than at the clinic. The site of semen collection does not affect the results of a semen analysis.[12]

Measurement methods

Computer Assisted Semen Analysis (CASA) is a catch-all phrase for automatic or semi-automatic semen analysis techniques. Most systems are based on image analysis, but alternative methods exist such as tracking cell movement on a digitizing tablet.[13][14] Computer-assisted techniques are most-often used for the assessment of sperm concentration and mobility characteristics, such as velocity and linear velocity.

See also

References

  1. ^ a b c d e f g h i j Understanding Semen Analysis. Stonybrook, State University of New York (1999). Retrieved on 2007-08-05.
  2. ^ a b c d e f g h i j Essig, Maria G.; Edited by Susan Van Houten and Tracy Landauer, Reviewed by Martin Gabica and Avery L. Seifert (2007-02-20). Semen Analysis. Healthwise. WebMD. Retrieved on 2007-08-05.
  3. ^ a b Ellington, Joanna (2004). Understanding a Sperm Analysis. INGfertility. Retrieved on 2007-08-05.
  4. ^ a b Dr. Joanna Ellington (January 2005). "Use of a Specialized Condom to Collect Sperm Samples for Fertility Procedures". INGfertility. Retrieved on 2007-08-05.
  5. ^ Kippley, John; Sheila Kippley (1996). The Art of Natural Family Planning, 4th addition, Cincinnati, OH: The Couple to Couple League, pp.306-307. ISBN 0-926412-13-2. 
  6. ^ Fertility Center, Stockholm (translated from Swedish)
  7. ^ Rajmil O, Fernández M, Rojas-Cruz C, Sevilla C, Musquera M, Ruiz-Castañe E (2007). "Azoospermia should not be given as the result of vasectomy" (in Spanish; Castilian). Arch. Esp. Urol. 60 (1): 55-8. PMID 17408173.
    Dhar NB, Bhatt A, Jones JS (2006). "Determining the success of vasectomy". BJU Int. 97 (4): 773-6. doi:10.1111/j.1464-410X.2006.06107.x. PMID 16536771.
  8. ^ Semen analysis morphology. IVF 1 (2005-10-27). Retrieved on 2007-08-05.
  9. ^ Ellington, Joanna (2004). How Long to Abstain for a Sperm Test/Analysis. INGfertility. Retrieved on 2007-08-06.
  10. ^ Weschler, Toni (2002). Taking Charge of Your Fertility, Revised Edition, New York: HarperCollins, p.189. ISBN 0-06-093764-5. 
  11. ^ Adequate Analysis Frequency. Kokopelli Technologies (2007). Retrieved on 2007-08-11.
  12. ^ Shetty Licht R, Handel L, Sigman M (2007). "Site of semen collection and its effect on semen analysis parameters". Fertil Steril.. doi:10.1016/j.fertnstert.2007.02.033. PMID 17482174.
  13. ^ Mortimer ST (2000). "CASA--practical aspects". J. Androl. 21 (4): 515-24. PMID 10901437. Retrieved on 2007-08-05.
  14. ^ Hinting A, Schoonjans F, Comhaire F (1988). "Validation of a single-step procedure for the objective assessment of sperm motility characteristics". Int. J. Androl. 11 (4): 277-87. PMID 3170018.
 
This article is licensed under the GNU Free Documentation License. It uses material from the Wikipedia article "Semen_analysis". A list of authors is available in Wikipedia.
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