To use all functions of this page, please activate cookies in your browser.
With an accout for my.bionity.com you can always see everything at a glance – and you can configure your own website and individual newsletter.
- My watch list
- My saved searches
- My saved topics
- My newsletter
Additional recommended knowledge
Like the two-hybrid system, phage display is used for the high-throughput screening of protein interactions. The principle of this method is based on the display of each DNA fragment from a relevant library from the C-terminus of phage-coat protein pIV. The vector can be transfected with helper phage into bacterial cells with a multiplicity of infection of 1 (i.e. each bacterial cell is infected with no more than one phage) to produce DNA-containing phage that display the relevant protein fragment as part of their outer coat. Via multiple cloning sites, the fragments are inserted in all three possible frames to ensure that the cDNA fragment is translated in the proper frame.
By immobilising a relevant [DNA or protein] target (or targets) to the surface of a well, a phage that displays a protein that binds to one of those targets on its surface, will remain while others are removed by washing. Those that remain can be eluted, used to produce more phage (by bacterial infection with helper phage) and so produce a phage mixture that is enriched with relevant (i.e. binding) phage. The repeated cycling of these steps is referred to as 'panning', in reference to the enrichment of a sample of gold by removing undesirable materials.
Phage eluted in the final step can be used to infect a suitable bacterial host, from which the phagemids can be collected and the relevant DNA sequence excised and sequenced to identify the relevant, interacting proteins or protein fragments.
Recent work published by Chasteen et al., shows that use of the helper phage can be eliminated by using a novel 'bacterial packaging cell line' technology.
The applications of this technology include determination of interaction partners of a protein (which would be used as the immobilised phage "bait" with a DNA library consisting of all coding sequences of a cell, tissue or organism) so that new functions or mechanisms of function of that protein may be inferred. The technique is also used to determine tumour antigens (for use in diagnosis and therapeutic targetting) and in searching for protein-DNA interactions using specially-constructed DNA libraries with randomised segments.
Phage display is also a widely used method for in vitro protein evolution (also called protein engineering). Competing methods for in vitro protein evolution are yeast display, bacterial display, ribosome display, and mRNA display.
Chasteen L, Ayriss J, Pavlik P, Bradbury AR. Eliminating helper phage from phage display. Nucleic Acids Res. 2006;34(21):e145. Epub 2006 Nov 6.
|This article is licensed under the GNU Free Documentation License. It uses material from the Wikipedia article "Phage_display". A list of authors is available in Wikipedia.|