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42 Current white paper about the topic fluorescence

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Transfection of Human Tooth-Specific Lineage Cells with FuGENE® HD Transfection Reagent

22-08-2008

Similar to other epithelial appendages, teeth form through reciprocal signaling interactions between the ectodermally derived dental epithelium and the mesenchyme. These signaling interactions program enamel matrix-secreting ameloblasts and dentin-forming odontoblasts. To study the signaling ...

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Interference and HAMA problems

Recognizing and avoiding of false results

16-06-2008

Immunoassays are bioanalytical methods based on antibodies. These include ELISA, EIA, RIA, Western blotting, protein arrays, lateral flow assays (immuno-chromatography), immunohistochemistry or immuno-PCR. Antibodies detect the substances (analytes) in immunoassays. This detection is always ...

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Transcriptor High Fidelity cDNA Synthesis Kit - Accuracy Meets RT-PCR

04-06-2008

Retroviral reverse transcriptases commonly used for cDNA synthesis exhibit a higher error rate than other DNA polymerases used in nucleic acid analysis techniques. This lack of accuracy leads to a significant number of base exchanges or frameshifts which are further propagated in a subsequent PCR ...

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Optimization of Transfection Conditions for Gene Expression, siRNA Knock-Down, and Live Cell Imaging Using FuGENE® HD Transfection Reagent

22-05-2008

The experimental method of introducing foreign mole­cules into cells is an important tool to facilitate studies in various biological fields. Transfection using lipophilic methods (liposomes or other cationic species) is a convenient means of introducing many different types of molecules (plasmid ...

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Molecular Probes® NanoOrange® Assay Performed on BMG LABTECH FLUOstar OPTIMA Microplate Reader

08-05-2008

The field of proteomics has expanded dramatically in recent years with research on a whole host of organisms. Techniques such as two dimensional difference gel electrophoresis (2D DIGE) require the accurate quantitation of protein, as up to three labelled samples can be loaded on a single gel for ...

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High sensitive detection of follistatin by Imperacer®: an initial study on the way to analytical gene-doping tests

08-05-2008

For the understanding of interactions in the muscle-growth directing myostatin-pathway and therefore also for the establishment of high sensitive detection strategies for gene-doping, detailed studies of protein concentration levels in biological matrices and appropriate highly sensitive analytic ...

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Rapid High-Throughput Methylation Analysis Using the LightCycler® 480 System

07-05-2008

The LightCycler® 480 Instrument is a versatile platform for molecular studies. We assessed the performance of the instrument combined with the LightCycler® 480 High Resolution Melting Master mix for DNA methylation analysis using the methylation-sensitive high resolution melting (MS-HRM) ...

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Dual Luciferase Reporter (DLR) Assay Certification on the Omega Series of Readers

29-02-2008

The Dual-Luciferase® Reporter Assay or DLR is widely used to study gene transcription and regulation. The DLR assay is a two step reaction that uses two luciferase enzymes, Firefly and Renilla. The Firefly reaction is initiated, followed by its quenching and the subsequent initiation of the ...

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Confocal Detection of NBT/BCIP In Situ Hybridization

Samples by Reflection Microscopy

18-02-2008

Confocal laser scanning reflection microscopy (CLSM) in combination with whole-mount in situ hybridization (WMISH) is a novel method to visualize gene expression patterns in three dimensions in whole embryos. The method uses conventional NBT/BCIP precipitation by alkaline phosphatase in WMISH ...

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Efficient Transfection of 293T Cell Line with Lentiviral Vectors Using FuGENE® HD Transfection Reagent

03-10-2007

Lentiviral vectors have become a powerful tool for gene transfer and genetic manipulation in research. Efficiency of the transfection reagent is most critical to getting a high-titer lentiviral recombinant virus. Here, we show that FuGENE® HD Transfection Reagent can be used to transfect 293T ...

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