22-08-2008

Introduction Similar to other epithelial appendages, teeth form through reciprocal signaling interactions between the ectodermally derived dental epithelium and the mesenchyme. These signaling interactions program enamel matrix-secreting ameloblasts and dentin-forming odontoblasts. To study ...

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Recognizing and avoiding of false results

16-06-2008

Immunoassays are bioanalytical methods based on antibodies. These include ELISA, EIA, RIA, Western blotting, protein arrays, lateral flow assays (immuno-chromatography), immunohistochemistry or immuno-PCR. Antibodies detect the substances (analytes) in immunoassays. This detection is always ...

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04-06-2008

New Enzyme Class Retroviral reverse transcriptases commonly used for cDNA synthesis exhibit a higher error rate than other DNA polymerases used in nucleic acid analysis techniques. This lack of accuracy leads to a significant number of base exchanges or frameshifts which are further ...

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22-05-2008

Introduction The experimental method of introducing foreign mole­cules into cells is an important tool to facilitate studies in various biological fields. Transfection using lipophilic methods (liposomes or other cationic species) is a convenient means of introducing many different types of ...

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08-05-2008

Abstract: For the understanding of interactions in the muscle-growth directing myostatin-pathway and therefore also for the establishment of high sensitive detection strategies for gene-doping, detailed studies of protein concentration levels in biological matrices and appropriate highly ...

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08-05-2008

NanoOrange® Assay for protein quantitation performed on the FLUOstar OPTIMA High and small concentration range evaluated Kit can easily be adapted for high throughput measurements #img4388# Introduction The field of proteomics has expanded dramatically in recent years with research on a ...

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07-05-2008

The LightCycler® 480 Instrument is a versatile platform for molecular studies. We assessed the performance of the instrument combined with the LightCycler® 480 High Resolution Melting Master mix for DNA methylation analysis using the methylation-sensitive high resolution melting (MS-HRM) ...

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29-02-2008

Promega’s DLR assay has been validated on the LUMIstar Omega, FLUOstar Omega, and POLARstar Omega Two onboard reagent injectors with simultaneous measurement allow for instantaneous detection of Firefly and Renilla luciferase Introduction The Dual-Luciferase® Reporter Assay or DLR is ...

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Samples by Reflection Microscopy

18-02-2008

Confocal laser scanning reflection microscopy (CLSM) in combination with whole-mount in situ hybridization (WMISH) is a novel method to visualize gene expression patterns in three dimensions in whole embryos. The method uses conventional NBT/BCIP precipitation by alkaline phosphatase in WMISH ...

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03-10-2007

Lentiviral vectors have become a powerful tool for gene transfer and genetic manipulation in research. Efficiency of the transfection reagent is most critical to getting a high-titer lentiviral recombinant virus. Here, we show that FuGENE® HD Transfection Reagent can be used to transfect 293T ...

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