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10 Current white paper about the topic biochemistryrss
Thioflavin T aggregation assay to determine antibody stability using the FLUOstar Omega
The efficiency of antibody molecules is dependent on their stability. We introduce an assay setup where stability of antibodies is measured. Shaking stress is initiated by the FLUOstar Omega microplate reader and aggregation propensity of antibody candidates is measured in 96-well format
Smart Solutions for large Molecules
As demonstrated here, there are a broad range of HPLC applications used in development, production, validation and release of protein therapeutics for human use. Some of the applications mentioned above are mandatory when submitting a new biopharmaceutical to approval at the regulatory agencies. ...
Polymer-based chromatographic resins featuring high mechanical and chemical stability and high protein binding capacities can help to increase the throughput and robustness of biopharmaceutical manufacturing processes. Advances in genetic engineering and cell culture technology have raised ...
The POLARstar Omega microplate reader from BMG LABTECH was used to study membrane fluidity via fluorescence polarization in 96-well format. The method allows for the monitoring of large scale alterations in membrane fluidity (such as phase transitions), as well as more subtle changes in lipid ...
Our results suggest that the influence of androgen receptor-stimulating or -blocking substances strongly depends upon the composition of the growth medium. The use of hormone-depleted FBS in combination with phenol red-free medium is strongly recommended for monitoring stimulator effects, whereas ...
As drugs applied to organisms are metabolized via the liver, hepatocytes represent a useful and common study model. Primary hepatocytes are widely used to evaluate the toxic effects of drugs and xenobiotics. A variety of metabolic parameters have been described to analyze the functionality of ...
Transfection is the delivery of DNA, RNA, proteins, and macromelecules into eukaryotic cells. The goals for transfection include the study of gene function and regulation as well as protein expression and function. It is also used for gene silencing and protein production. According to the ...
For the in vitro testing of pharmaceutical, cosmetic and chemical products conventional cell cultures are increasingly being replaced by three-dimensional (3D) cell culture models with organ-specific characteristics. Using tissue engineering techniques, a living human skin equivalent has been ...
Determining the protein concentration of samples is a necessary and often used method in biochemistry. Different colorimetric protein assays have been developed. The most commonly used methods are the Bradford assay, the Lowry assay and the BCA assay. In this application note we demonstrate how ...