Thioflavin T aggregation assay to determine antibody stability using the FLUOstar Omega

03-11-2011

Stress can cause denaturation and aggregation of antibodies Aggregation propensity of antibody candidates was measured using a fluorescence based assay in 96-well format Shaking stress initiated by the FLUOstar Omega and the addition of Teflon beads enabled a successful antibody ...

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Smart Solutions for large Molecules

24-11-2010

Development and production of biopharmaceuticals is a growing segment of pharmaceutical industry. Therapeutic proteins include blood factors, thrombolytic agents, growth factors, interferons, interleukins, therapeutic enzymes and last but not least monoclonal antibodies. More than twenty ...

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16-11-2010

Polymer-based chromatographic resins featuring high mechanical and chemical stability and high protein binding capacities can help to increase the throughput and robustness of biopharmaceutical manufacturing processes. Advances in genetic engineering and cell culture technology have raised ...

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23-06-2010

Membrane fluidity is measured in both liposomes and mitochondrial membranes via fluorescence polarization UV FP optics are used on the POLARstar Omega to measure DPH (1,6-diphenyl-hexa-1,3,5-triene) fluorescence The effects of dibucaine and propranolol on membrane fluidity are ...

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11-01-2010

We used a novel real-time method to analyze the cellular growth of the prostate cancer cell line LNCaP under different conditions. This method allowed us to determine the optimal time points for adding substances that affect cell growth, and reduced the likelihood of missing transient ...

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07-12-2009

Introduction As drugs applied to organisms are metabolized via the liver, hepatocytes represent a useful and common study model. Primary hepatocytes are widely used to evaluate the toxic effects of drugs and xenobiotics. A variety of metabolic parameters have been described to analyze the ...

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31-08-2009

General: Definitions and Techniques FAQ: What is transfection and what are the commonly used methods? HelpCorner: Transfection is the delivery of DNA, RNA, proteins, and macromelecules into eukaryotic cells. The goals for transfection include the study of gene function and regulation as ...

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05-06-2009

Introduction For the in vitro testing of pharmaceutical, cosmetic and chemical products conventional cell cultures are increasingly being replaced by three-dimensional (3D) cell culture models with organ-specific characteristics. Using tissue engineering techniques, a living human skin ...

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28-02-2008

Fast and homogeneous assay to determine the protein concentration of samples Dye shift can be followed with the spectrometer tool in the new FLUOstar Omega New easy to use Omega evaluation software is introduced #img4365# Introduction Determining the protein concentration of samples is a ...

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29-09-2004

The success of chromatography as a technique for separating biomolecules is inevitably linked to the introduction of the gel filtration medium SephadexTM by Pharmacia AB in 1959. The now classical paper by Porath and Flodin [1] not only described one of the most significant advances ever made ...

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