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118 Current white paper of Roche Diagnostics
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Isolation of DNA from Dried Blood Spot Using the High Pure PCR Template Preparation Kit
07-12-2009
Introduction DNA isolation from anti-coagulated whole blood for subsequent molecular analysis by PCR methods is well-established. In a standard laboratory setting, the blood is shipped in a blood collection vial containing the anticoagulant after phlebotomy. However, it might not always be ...
Increased Vitality of Primary Mouse Hepatocytes Isolated with Liberase TM Research Grade
07-12-2009
Introduction As drugs applied to organisms are metabolized via the liver, hepatocytes represent a useful and common study model. Primary hepatocytes are widely used to evaluate the toxic effects of drugs and xenobiotics. A variety of metabolic parameters have been described to analyze the ...
Array Capture-mediated Resequencing Leads to the Discovery of Four Novel Mutations in the Mouse
02-12-2009
By applying array-based sequence capture and pyrosequencing, D’Ascenzo and co-workers successfully identified one known (positive control) and four previously unknown mutations in the mouse Kit gene. These data represent the first documentation and validation of the fact that the new ...
Acquired Copy Number Alterations in Adult Acute Myeloid Leukemia Genomes
30-11-2009
Cytogenetic analysis of acute myeloid leukemia (AML) cells has accelerated the identification of genes important for AML pathogenesis. As shown in a recent scientific study, the use of an unbiased high-resolution genomic screen yields the identification of genes altered in AML genomes not ...
Amplicon Sequencing with the GS FLX Titanium Series: High Sensitivity Targeted Resequencing
30-11-2009
Introduction The power of the Genome Sequencer FLX System and its utility for sequencing amplicons derive from the ability to sequence from single molecules within a mixture of molecules. In this process, each amplicon molecule, also known as a PCR product, is sequenced individually, ...
Adaptation of SmartAmp-2 Assays for Microsatellite Copy Number Polymorphism Detection on the LightCycler® 480 Instrument
07-09-2009
Introduction The Smart Amplification process, version 2 (SMAP-2), also known as SmartAmp-2, is a rapid polymorphism detection system based on a unique asymmetric primer design, including a folding primer (FP) and a turn-back primer (TP) [1]. Combined with Thermus aquaticus MutS (Taq MutS) ...
High-Throughput Analysis Using the Novel LightCycler® 1536 Real-Time PCR System
07-09-2009
Introduction An important trend in biological and biochemical analysis over the past 15 years has been miniaturization and parallelization of analytical procedures. Large-scale high-throughput analysis of gene expression or genetic polymorphisms is an essential element of modern functional ...
HelpCorner - Transfection
31-08-2009
General: Definitions and Techniques FAQ: What is transfection and what are the commonly used methods? HelpCorner: Transfection is the delivery of DNA, RNA, proteins, and macromelecules into eukaryotic cells. The goals for transfection include the study of gene function and regulation as ...
New Products for Cell Culture Analysis from Roche Innovatis
31-08-2009
The Roche Innovats product line is now available from Roche Applied Sciences. Below is a short introduction to three Roche Innovatis product lines designed for the complete analysis of cell cultures, from the simple determination of cell concentration to complex cellular screening. More ...
Efficient Reverse Transfection of Both siRNA and Expression Plasmids on Transfected Cell Microarrays Using X-tremeGENE siRNA Transfection Reagent
24-08-2009
Introduction Transfected cell microarray is a miniaturization of cell-based functional studies and allows spatially restricted transfection without the use of wells [1]. Expression plasmids and/or siRNAs complexed with a transfection reagent in a gel are printed on a glass slide. Cells ...
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